Rhesus D peptides that stimulate peripheral blood mononuclear cells from deliberately alloimmunized RhD-negative plasma donors to proliferate in vitro
| Donor . | Sex . | HLA-DR type . | Serum anti-D antibody (IU/mL*) + other antibody . | Deliberate immunizations with RhD+RBCs . | RhD peptides stimulating proliferation† . | |
|---|---|---|---|---|---|---|
| No. times/type RBCs . | Date of most recent immunization . | |||||
| 1 | M | DRB1*15;11 | 699 + C‡ + E | 5/R2 cells | 7/1996 | 2, 3, 4, 5, 6, 10, 13, 18, 21, 26, 28, 40, 42, 43, 44, 47, 52, 54, 68 |
| 2 | M | DRB1*1;11 | 462 + C + E | 7/NK | 9/1994 | 5,8, 10, 12, 17, 46, 63 |
| 3 | M | DRB1*15;7 | 37 + C | 5/NK | 7/1996 | 13, 28, 63 |
| 4 | M | DRB1*3;11 | 1190 + C‡ + E | 6/R2 cells | 7/1997 | 3, 5, 6, 7, 9, 12, 13, 17, 22, 25, 27, 29, 33, 40,46, 47, 49, 51, 52, 53, 55, 59, 63, 65, 66, 67, 68 |
| 5 | M | DRB1*3;7 | 3 + C + E | 3/R1R2 cells | 6/1997 | 16 |
| 6 | M | DRB1*15 | 196 + C‡ + E | 6/R2 cells | 7/1997 | 1, 25, 6, 7, 8, 9, 10, 13, 14, 17, 19, 22, 24, 25, 26, 27, 28, 37, 44, 46, 47, 51, 53, 59, 61, 63,64, 67, 68 |
| 7 | M | DRB1*7;13 | 304 + C | 5/R1 cells | 7/1997 | 4,17, 52 |
| 8 | M | DRB1*15;11 | 2724 + C‡ + E | 4/R2 cells | 8/1997 | 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20, 22, 23, 24, 26, 27, 28, 32, 37, 39, 40, 44, 45,46, 48, 49, 52, 54, 55, 56, 58, 59, 61, 62, 63,66, 67 |
| 9 | F | DRB1*15;7 | 215 + C‡ + E | 5/R2 cells | 7/1994 | 53 |
| 10 | F | DRB1*15 | 590 + C | 9/R1 cells | 7/1997 | 5,6, 10, 11, 17, 27 |
| 11 | F | DRB1*7 | 128 + C | 4/R1 cells | 7/1997 | 9, 13, 16, 17, 26, 28, 46, 47, 63, 68 |
| 12 | F | DRB1*15;13 | 699 + C‡ | 3/R2 cells | 8/1993 | 2, 3, 6, 7, 9, 10, 11, 13, 14, 15, 16, 17, 19, 20,21, 22, 24, 26, 27, 28, 29, 30, 32, 35, 36, 38, 40, 41, 45,46, 47, 48, 49, 51, 53, 55, 56, 58, 59, 60, 61, 62, 63,64 |
| 13 | F | DRB1*15 | 1528 + C | 2/R1 cells | 7/1994 | 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 20, 21, 22, 23, 26, 27, 28, 30, 33, 38, 39, 40, 41, 44, 45, 46, 48, 49, 54, 55, 56, 57, 58, 61, 62, 63, 67, 68 |
| Donor . | Sex . | HLA-DR type . | Serum anti-D antibody (IU/mL*) + other antibody . | Deliberate immunizations with RhD+RBCs . | RhD peptides stimulating proliferation† . | |
|---|---|---|---|---|---|---|
| No. times/type RBCs . | Date of most recent immunization . | |||||
| 1 | M | DRB1*15;11 | 699 + C‡ + E | 5/R2 cells | 7/1996 | 2, 3, 4, 5, 6, 10, 13, 18, 21, 26, 28, 40, 42, 43, 44, 47, 52, 54, 68 |
| 2 | M | DRB1*1;11 | 462 + C + E | 7/NK | 9/1994 | 5,8, 10, 12, 17, 46, 63 |
| 3 | M | DRB1*15;7 | 37 + C | 5/NK | 7/1996 | 13, 28, 63 |
| 4 | M | DRB1*3;11 | 1190 + C‡ + E | 6/R2 cells | 7/1997 | 3, 5, 6, 7, 9, 12, 13, 17, 22, 25, 27, 29, 33, 40,46, 47, 49, 51, 52, 53, 55, 59, 63, 65, 66, 67, 68 |
| 5 | M | DRB1*3;7 | 3 + C + E | 3/R1R2 cells | 6/1997 | 16 |
| 6 | M | DRB1*15 | 196 + C‡ + E | 6/R2 cells | 7/1997 | 1, 25, 6, 7, 8, 9, 10, 13, 14, 17, 19, 22, 24, 25, 26, 27, 28, 37, 44, 46, 47, 51, 53, 59, 61, 63,64, 67, 68 |
| 7 | M | DRB1*7;13 | 304 + C | 5/R1 cells | 7/1997 | 4,17, 52 |
| 8 | M | DRB1*15;11 | 2724 + C‡ + E | 4/R2 cells | 8/1997 | 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20, 22, 23, 24, 26, 27, 28, 32, 37, 39, 40, 44, 45,46, 48, 49, 52, 54, 55, 56, 58, 59, 61, 62, 63,66, 67 |
| 9 | F | DRB1*15;7 | 215 + C‡ + E | 5/R2 cells | 7/1994 | 53 |
| 10 | F | DRB1*15 | 590 + C | 9/R1 cells | 7/1997 | 5,6, 10, 11, 17, 27 |
| 11 | F | DRB1*7 | 128 + C | 4/R1 cells | 7/1997 | 9, 13, 16, 17, 26, 28, 46, 47, 63, 68 |
| 12 | F | DRB1*15;13 | 699 + C‡ | 3/R2 cells | 8/1993 | 2, 3, 6, 7, 9, 10, 11, 13, 14, 15, 16, 17, 19, 20,21, 22, 24, 26, 27, 28, 29, 30, 32, 35, 36, 38, 40, 41, 45,46, 47, 48, 49, 51, 53, 55, 56, 58, 59, 60, 61, 62, 63,64 |
| 13 | F | DRB1*15 | 1528 + C | 2/R1 cells | 7/1994 | 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 20, 21, 22, 23, 26, 27, 28, 30, 33, 38, 39, 40, 41, 44, 45, 46, 48, 49, 54, 55, 56, 57, 58, 61, 62, 63, 67, 68 |
Peptides containing Rhesus D–specific polymorphisms are in boldface. The results are combined if more than one sample was tested.
RhD indicates Rhesus D; RBCs, red blood cells; NK, not known.
Quantified against national reference standards; if tested more than once, highest level is shown.
Peptides 34, 35, and 36 containing Met218 were not tested in donors 1-5, 7, 9, or 13.
The presence of anti-C when given only R2 cells indicates anti-G.