Lineage analysis of and GFP expression in bone marrow cells from primary and secondary recipient NOD/SCID mice
| . | Engraftment in recipients (%) . | GFP expression in recipients (%) . | ||
|---|---|---|---|---|
| Primary . | Secondary . | Primary . | Secondary . | |
| CD34 | 26 ± 2 | 10 ± 5 | 9 ± 2 | 27 ± 9 |
| CD38 | 81 ± 17 | 67 ± 10 | 8 ± 2 | 22 ± 8 |
| CD33 | 4 ± 2 | 14 ± 4 | 14 ± 3 | 39 ± 5 |
| CD19 | 32 ± 6 | 40 ± 8 | 9 ± 1 | 19 ± 3 |
| CD14 | 3 ± 1 | 15 ± 2 | 14 ± 6 | 23 ± 5 |
| . | Engraftment in recipients (%) . | GFP expression in recipients (%) . | ||
|---|---|---|---|---|
| Primary . | Secondary . | Primary . | Secondary . | |
| CD34 | 26 ± 2 | 10 ± 5 | 9 ± 2 | 27 ± 9 |
| CD38 | 81 ± 17 | 67 ± 10 | 8 ± 2 | 22 ± 8 |
| CD33 | 4 ± 2 | 14 ± 4 | 14 ± 3 | 39 ± 5 |
| CD19 | 32 ± 6 | 40 ± 8 | 9 ± 1 | 19 ± 3 |
| CD14 | 3 ± 1 | 15 ± 2 | 14 ± 6 | 23 ± 5 |
Multilineage engraftment of human cells and expression of GFP in the bone marrow of primary and secondary NOD/SCID recipient mice transplanted with lentivirally transduced cord blood CD34+cells. Bone marrow from 6 mice from groups III and IV were stained with the following human specific monoclonal antibodies: CD34, CD38, CD33, CD19, and CD14 and analyzed by flow cytometry. For all antibodies the negative control used was IgG1. Double staining with CD45 was done with each lineage marker and the fraction of GFP-expressing cells in the varying lineages was assessed. The results are presented as mean ± SD.