Summary of publications on Epstein-Barr virus load in healthy carriers and patients with post-transplantation lymphoproliferative disease
| PCR method . | Copy number of EBV genomes per 106PBMCs7-150 . | Reference . | |
|---|---|---|---|
| Healthy carriers . | Patients with PTLD . | ||
| Quantitative competitive | ND to 1921 (13/13) | 2930 to 72 537 (8/8) | Current study |
| Quantitative competitive | ND to 20 (10/10) | 5000 to > 250 000 (13/14), 200 (1/14) | 14 |
| Quantitative competitive | — | 3000 to 40 000 (7/7) | 15 |
| Semiquantitative | ND to 25 (4/4) | 5000 to 5 000 000 (10/10) | 16 |
| Semiquantitative | 200 to 20 000 (10/10) | > 200 000 (5/7), < 20 000 (2/7) | 18 |
| Semiquantitative | ND to 5 (16/16) | N/A | 32 |
| Semiquantitative | ND (5/10), 20 000 to 1 000 000 (5/10) | N/A | 33 |
| Real-time | ND to 675 (13/13) | 2890 to 134 455 (5/5) | 34 |
| PCR method . | Copy number of EBV genomes per 106PBMCs7-150 . | Reference . | |
|---|---|---|---|
| Healthy carriers . | Patients with PTLD . | ||
| Quantitative competitive | ND to 1921 (13/13) | 2930 to 72 537 (8/8) | Current study |
| Quantitative competitive | ND to 20 (10/10) | 5000 to > 250 000 (13/14), 200 (1/14) | 14 |
| Quantitative competitive | — | 3000 to 40 000 (7/7) | 15 |
| Semiquantitative | ND to 25 (4/4) | 5000 to 5 000 000 (10/10) | 16 |
| Semiquantitative | 200 to 20 000 (10/10) | > 200 000 (5/7), < 20 000 (2/7) | 18 |
| Semiquantitative | ND to 5 (16/16) | N/A | 32 |
| Semiquantitative | ND (5/10), 20 000 to 1 000 000 (5/10) | N/A | 33 |
| Real-time | ND to 675 (13/13) | 2890 to 134 455 (5/5) | 34 |
PCR indicates polymerase chain reaction; PBMC, peripheral blood mononuclear cell; PTLD, post-transplantation lymphoproliferative disease; ND, not detected.
Data from original papers are normalized to copy number of Epstein-Barr virus genomes per 106 PBMCs for comparison. In studies where the original data are presented as copy number per 106 B cells or copy number per μg DNA, the values are normalized by estimating that B cells account for 10% of PBMCs or that 1 μg DNA corresponds to 0.2 × 106 PBMCs, respectively. Fractions in parentheses represent number of cases within that range (nominator) and total number of cases measured (denominator).