Oligonucleotide primers and probes used in reverse transcription polymerase chain reaction analysis
| Transcript . | Primer or probe designation . | Genome coordinates in B95.8 . | Annealing temperature . | Oligonucleotide sequence . | Reference . |
|---|---|---|---|---|---|
| EBER1 | 5′ primer | 6776-6795 | 58°C | 5′-AAAACATGCGGACCACCAGC | 9 |
| 3′ primer | 6648-6629 | 5′-AGGACCTACGCTGCCCTAGA | |||
| Probe | 6718-6737 | 5′-ACGGTGTCTGTGGTTGTCTT | |||
| EBNA1 | 5′ primer (Q exon) | 62440-62457 | 55°C | 5′-GTGCGCTACCGGATGGCG | 9 |
| 5′ primer (U exon) | 67483-67502 | 5′-TTAGGAAGCGTTTCTTGAGC | |||
| 3′ primer (K exon) | 107986-107967 | 5′-CATTTCCAGGTCCTGTACCT | |||
| Probe | 67544-67563 | 5′-AGAGAGTAGTCTCAGGGCAT | |||
| EBNA2 | 5′ primer | 14802-14822 | 60°C | 5′-AGAGGAGGTGGTAAGCGGTTC | 9, 26 |
| 3′ primer | 48583-48562 | 5′-TGACGGGTTTCCAAGACTATCC | |||
| Probe | 48397-48416 | 5′-TGGCGTGTGACGTGGTGTAA | |||
| LMP2A | 5′ primer | 166824-166843 | 57°C | 5′-GCAACACGACGGGAATGACG | 10 |
| 3′ primer | 131-112 | 5′-AAACACGAGGCGGCAATAGC | |||
| Probe | 62-81 | 5′-ATCCAGTATGCCTGCCTGTA | |||
| BZLF1 | 5′ primer | 102826-102807 | 58°C | 5′-GGGAGAAGCACCTCAACCTG | 26 |
| 3′ primer | 102447-102466 | 5′-TTGCTTAAACTTGGCCCGGC | |||
| Probe | 102665-102656/102530-102521 | 5′-AGCCAGAATC/CTGGAGGAAT | |||
| BLLF1 | 5′ primer | 89934-89955 | 63°C | 5′-GTGGATGTGGAACTGTTTCCAG | 26 |
| 3′ primer | 90753-90732 | 5′-CTGTATCCACCGCGGATGTCAC | |||
| Probe | 90682-90663 | 5′-AGTCCATCTCCATGGGACAA | |||
| BART | 5′ primer | 157154-157173 | 55°C | 5′-AGAGACCAGGCTGCTAAACA | 27 |
| 3′ primer | 159194-159175 | 5′-AACCAGCTTTCCTTTCCGAG | |||
| Probe | 157359-157378 | 5′-AAGACGTTGGAGGCACGCTG |
| Transcript . | Primer or probe designation . | Genome coordinates in B95.8 . | Annealing temperature . | Oligonucleotide sequence . | Reference . |
|---|---|---|---|---|---|
| EBER1 | 5′ primer | 6776-6795 | 58°C | 5′-AAAACATGCGGACCACCAGC | 9 |
| 3′ primer | 6648-6629 | 5′-AGGACCTACGCTGCCCTAGA | |||
| Probe | 6718-6737 | 5′-ACGGTGTCTGTGGTTGTCTT | |||
| EBNA1 | 5′ primer (Q exon) | 62440-62457 | 55°C | 5′-GTGCGCTACCGGATGGCG | 9 |
| 5′ primer (U exon) | 67483-67502 | 5′-TTAGGAAGCGTTTCTTGAGC | |||
| 3′ primer (K exon) | 107986-107967 | 5′-CATTTCCAGGTCCTGTACCT | |||
| Probe | 67544-67563 | 5′-AGAGAGTAGTCTCAGGGCAT | |||
| EBNA2 | 5′ primer | 14802-14822 | 60°C | 5′-AGAGGAGGTGGTAAGCGGTTC | 9, 26 |
| 3′ primer | 48583-48562 | 5′-TGACGGGTTTCCAAGACTATCC | |||
| Probe | 48397-48416 | 5′-TGGCGTGTGACGTGGTGTAA | |||
| LMP2A | 5′ primer | 166824-166843 | 57°C | 5′-GCAACACGACGGGAATGACG | 10 |
| 3′ primer | 131-112 | 5′-AAACACGAGGCGGCAATAGC | |||
| Probe | 62-81 | 5′-ATCCAGTATGCCTGCCTGTA | |||
| BZLF1 | 5′ primer | 102826-102807 | 58°C | 5′-GGGAGAAGCACCTCAACCTG | 26 |
| 3′ primer | 102447-102466 | 5′-TTGCTTAAACTTGGCCCGGC | |||
| Probe | 102665-102656/102530-102521 | 5′-AGCCAGAATC/CTGGAGGAAT | |||
| BLLF1 | 5′ primer | 89934-89955 | 63°C | 5′-GTGGATGTGGAACTGTTTCCAG | 26 |
| 3′ primer | 90753-90732 | 5′-CTGTATCCACCGCGGATGTCAC | |||
| Probe | 90682-90663 | 5′-AGTCCATCTCCATGGGACAA | |||
| BART | 5′ primer | 157154-157173 | 55°C | 5′-AGAGACCAGGCTGCTAAACA | 27 |
| 3′ primer | 159194-159175 | 5′-AACCAGCTTTCCTTTCCGAG | |||
| Probe | 157359-157378 | 5′-AAGACGTTGGAGGCACGCTG |
See footnote to Table 5 for explanation of abbreviations.