Secondary transplantation of bone marrow cells from the recipients of T+ KSLA fetal liver cells
| Origins of the cells . | Time after transplantation, wk . | Positive mice . | % Chimerism . |
|---|---|---|---|
| T+KSLA | 4 | 3/3 | 16.1 ± 1.0 (n = 3) |
| 12 | 3/3 | 3.9 ± 2.1 (n = 3) | |
| Competitor | 4 | 6/6 | 15.4 ± 6.0 (n = 6) |
| 12 | 6/6 | 3.5 ± 1.9 (n = 6) |
| Origins of the cells . | Time after transplantation, wk . | Positive mice . | % Chimerism . |
|---|---|---|---|
| T+KSLA | 4 | 3/3 | 16.1 ± 1.0 (n = 3) |
| 12 | 3/3 | 3.9 ± 2.1 (n = 3) | |
| Competitor | 4 | 6/6 | 15.4 ± 6.0 (n = 6) |
| 12 | 6/6 | 3.5 ± 1.9 (n = 6) |
Primary recipients had transplantation with 20 T+KSLA cells of B6-F1, together with 2 × 105 bone marrow competitor cells of B6-Ly5.1. At 25 weeks after transplantation, the recipients presenting about 70% chimerism of either fetal liver– or competitor-derived cells were selected and used as donors. Bone marrow cells presenting F1 type or Ly5.1 type were independently sorted on a FACS, and 1.0 × 106 sorted cells of F1 or Ly5.1 type were transplanted into irradiated B6-Ly5.2 mice together with 2 × 105 freshly isolated bone marrow cells of B6-Ly5.2.