Transplantation of purified fetal liver cells at day 14 of gestation
| Cells . | Time after transplantation, wk . | Positive mice . | % Chimerism . | RU/100 . |
|---|---|---|---|---|
| 100 KSLA cells | 4 | 6/7 | 5.3 ± 6.8 (n = 6) | 0.10 |
| 12 | 7/7 | 11.4 ± 11.5 (n = 7) | 0.28 | |
| 24 | 7/7 | 13.4 ± 8.8 (n = 7) | 0.31 | |
| 10 T+ KSLA cells | 4 | 7/9 | 6.2 ± 5.5 (n = 7) | 1.08 |
| 12 | 7/9 | 15.6 ± 11.7 (n = 7) | 2.88 | |
| 24 | 7/9 | 21.4 ± 25.8 (n = 7) | 4.24 | |
| 100 T− KSLA cells | 4 | 2/7 | 1.5 (n = 2) | 0.009 |
| 12 | 1/7 | 1.1 (n = 1) | 0.003 | |
| 24 | 0/7 |
| Cells . | Time after transplantation, wk . | Positive mice . | % Chimerism . | RU/100 . |
|---|---|---|---|---|
| 100 KSLA cells | 4 | 6/7 | 5.3 ± 6.8 (n = 6) | 0.10 |
| 12 | 7/7 | 11.4 ± 11.5 (n = 7) | 0.28 | |
| 24 | 7/7 | 13.4 ± 8.8 (n = 7) | 0.31 | |
| 10 T+ KSLA cells | 4 | 7/9 | 6.2 ± 5.5 (n = 7) | 1.08 |
| 12 | 7/9 | 15.6 ± 11.7 (n = 7) | 2.88 | |
| 24 | 7/9 | 21.4 ± 25.8 (n = 7) | 4.24 | |
| 100 T− KSLA cells | 4 | 2/7 | 1.5 (n = 2) | 0.009 |
| 12 | 1/7 | 1.1 (n = 1) | 0.003 | |
| 24 | 0/7 |
Kit+, Sca+, Lin−/low, AA4.1+ (KSLA) cells were sorted on a FACS and transplanted to lethally irradiated adult mice together with 2 × 105 adult bone marrow cells. KSLA cells were fractionated based on Tie-2 expression. Tie-2–positive (T+) and –negative (T−) KSLA cells were also transplanted. After transplantation, peripheral blood cells of the recipients were analyzed to evaluate engraftment. The number of positive mice per number of mice having transplantation is shown. The mean of repopulating units (RU) per 100 cells (RU/100) was obtained with the formula of Harrison et al.10