Expansion of mitogen-stimulated human γδ-T cells is augmented by anti-CD2 mAb S5.2 in the presence of recombinant human IL-12
| Culture condition* . | Fold expansion γδ-T cells† . | |||
|---|---|---|---|---|
| IFN-γ . | Anti-CD2 . | IL-12 . | Mean ± SD (n = 5) . | P . |
| + | IgG2a | PBS | 17.7 ± 11.4 | |
| + | IgG2a | + | 20.9 ± 10.5 | NS |
| + | PBS | + | 23.0 ± 13.1 | NS |
| + | 0.1 μg/mL | + | 41.9 ± 20.3 | .08 |
| + | 1 μg/mL | + | 72.2 ± 60.8 | .10 |
| + | 5 μg/mL | + | 67.1 ± 33.7 | <.02 |
| Culture condition* . | Fold expansion γδ-T cells† . | |||
|---|---|---|---|---|
| IFN-γ . | Anti-CD2 . | IL-12 . | Mean ± SD (n = 5) . | P . |
| + | IgG2a | PBS | 17.7 ± 11.4 | |
| + | IgG2a | + | 20.9 ± 10.5 | NS |
| + | PBS | + | 23.0 ± 13.1 | NS |
| + | 0.1 μg/mL | + | 41.9 ± 20.3 | .08 |
| + | 1 μg/mL | + | 72.2 ± 60.8 | .10 |
| + | 5 μg/mL | + | 67.1 ± 33.7 | <.02 |
PBMC were obtained from 5 healthy donors; short-term cultures of PBMC from each donor were initiated, maintained, and analyzed for the fold expansion of γδ-T cells after equivalent culture durations.
On day 0, IFN-γ (1000 U/mL), anti-CD2 mAb S5.2 (indicated concentration, isotype control IgG2a, or PBS) and IL-12 (10 U/mL or PBS) were added to PBMC cultures (1 × 106cells/mL). Twenty-four hours later (day 1), all cultures were stimulated with OKT3 (10 ng/mL) and IL-2 (300 U/mL).
Data are means (±SD) from separate experiments performed using PBMC derived from 5 persons. P values are between indicated condition and control (IFN-γ + IgG2a + PBS), derived using a standard Student t test.