Synthetic oligonucleotides for PCR
| Primer . | Sequence . | Location . |
|---|---|---|
| ALAD 2 | 5′-GTTCTAGAGGGCCTGGCACTGTCCTCCA-3′ | 3′-untranslated |
| ALAD 3 | 5′-GCAATCCATCTGTTGAGGA-3′ | exon 5 |
| ALAD 4 | 5′-CACAGGCAGACATCACAGGCC-3′ | exon 5 |
| ALAD 17 | 5′-TACAGCCTATCACCAGCCTC-3′ | exon 3 |
| ALAD 21 | 5′-GGGAGACCGGAGCGGGAGACA-3′ | exon 1A |
| ALAD 23 | 5′-CAATGCCCCAGGAGCCCTCG-3′ | exon 2 |
| ALAD 29 | 5′-CTGTAGCTCATCACCGATAC-3′ | exon 8 |
| ALAD 31 | 5′-GGTTGTCACTTGTCTGAGGC-3′ | 3′-untranslated |
| Primer . | Sequence . | Location . |
|---|---|---|
| ALAD 2 | 5′-GTTCTAGAGGGCCTGGCACTGTCCTCCA-3′ | 3′-untranslated |
| ALAD 3 | 5′-GCAATCCATCTGTTGAGGA-3′ | exon 5 |
| ALAD 4 | 5′-CACAGGCAGACATCACAGGCC-3′ | exon 5 |
| ALAD 17 | 5′-TACAGCCTATCACCAGCCTC-3′ | exon 3 |
| ALAD 21 | 5′-GGGAGACCGGAGCGGGAGACA-3′ | exon 1A |
| ALAD 23 | 5′-CAATGCCCCAGGAGCCCTCG-3′ | exon 2 |
| ALAD 29 | 5′-CTGTAGCTCATCACCGATAC-3′ | exon 8 |
| ALAD 31 | 5′-GGTTGTCACTTGTCTGAGGC-3′ | 3′-untranslated |
ALAD cDNA or genomic DNA was amplified by PCR using a pair of synthetic oligonucleotides as primers, as described in “Materials and methods.”