Table 1. Transduction of cell lines Cell... | American Society of Hematology

Table 1.

Transduction of cell lines

Cell type	Culture condition	Percentage of GFP-positive cells
Cell type	Culture condition	MLV	Lenti
HeLa	Growing^*	79.5	60.0
	Contact inhibited^†	7.4	39.2
	Aphidicolin^§	1.8	13.5
3T3	Growing^*	34.0	24.5
	Contact inhibited^†	5.4	42.0
	Aphidicolin^§	0.2	11.5

Cell type	Culture condition	Percentage of GFP-positive cells
Cell type	Culture condition	MLV	Lenti
HeLa	Growing^*	79.5	60.0
	Contact inhibited^†	7.4	39.2
	Aphidicolin^§	1.8	13.5
3T3	Growing^*	34.0	24.5
	Contact inhibited^†	5.4	42.0
	Aphidicolin^§	0.2	11.5

Transductions were performed by placing a 1:5 dilution of the Moloney murine leukemia virus (MLV)-GFP or lenti-GFP supernatant over the cells for 24 hours.

Cells were plated in improved minimum essential medium (IMEM) and 10% fetal calf serum (FCS) for 48 hours before transduction and grown for 72 hours after transduction.

†

Cells were grown to confluence in IMEM-10% FCS, serum was then deprived with 0.1% FCS, and liquid media were supplemented for 72 hours before transduction. They were maintained for another 72 hours under the same conditions after transduction before analysis.

‡

Cells were grown in IMEM-10% FCS. After 48 hours, the medium was supplemented with aphidicolin 15 μg/mL 24 hours before, for the duration of transduction, and for 72 hours after transduction.

View Large

This Feature Is Available To Subscribers Only