Effects of various differentiation reagents on KG-1 cells
| Treatment . | NBT reduction (times control) . | NAE staining (% positive cells) . | FR-β (relative fluorescence) . |
|---|---|---|---|
| None | 1.00 ± 0 | 6.8 ± 0.34 | 1.7 ± 0.03 |
| ATRA* | 0.87 ± 0.04 | 6.2 ± 0.31 | 21.6 ± 0.05 |
| PMA | 0.93 ± 0.05 | 28.7 ± 1.43 | 0.90 ± 0.05 |
| DEX | 1.10 ± 0.05 | 6.2 ± 0.31 | 1.7 ± 0.08 |
| Vit D3 | — | — | 1.8 ± 0.01 |
| TGF-β | — | — | 1.4 ± 0.01 |
| Treatment . | NBT reduction (times control) . | NAE staining (% positive cells) . | FR-β (relative fluorescence) . |
|---|---|---|---|
| None | 1.00 ± 0 | 6.8 ± 0.34 | 1.7 ± 0.03 |
| ATRA* | 0.87 ± 0.04 | 6.2 ± 0.31 | 21.6 ± 0.05 |
| PMA | 0.93 ± 0.05 | 28.7 ± 1.43 | 0.90 ± 0.05 |
| DEX | 1.10 ± 0.05 | 6.2 ± 0.31 | 1.7 ± 0.08 |
| Vit D3 | — | — | 1.8 ± 0.01 |
| TGF-β | — | — | 1.4 ± 0.01 |
NBT indicates nitroblue tetrazolium; NAE, α-naphthyl acetate esterase; ATRA, all-trans retinoic acid; PMA, phorbol 12-myristate 13-acetate; DEX, dexamethasone; Vit D3, 1,25(OH)2 vitamin D3; and TGF, transforming growth factor. Dash indicates not determined.
HL60 cells were used as a positive control for ATRA-induced differentiation, giving an NBT reduction value of 4.3 ± 0.22 times control.