HLA class I and class II expression
| Antibody . | Extranodal DLCL* . | Nodal DLCL* % of cases (n = 21) . | Total extranodal vs nodal DLCL† . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Testicular % of cases (n = 28) . | CNS % of cases (n = 11) . | |||||||||
| − . | +/− . | + . | − . | +/− . | + . | − . | +/− . | + . | P . | |
| HLA-A | 61 | 29 | 11 | 55 | 18 | 27 | 10 | 33 | 57 | 0.008 |
| β2M‡ | 23 | 46 | 31 | 18 | 27 | 55 | 11 | 5 | 84 | 0.7 |
| TAP‡ | 26 | 33 | 41 | 30 | 40 | 30 | 0 | 9 | 91 | 0.02 |
| HLA-DR | 61 | 29 | 11 | 46 | 18 | 36 | 5 | 10 | 85 | 0.004 |
| W6/32‡ | 62 | 0 | 38 | 75 | 0 | 25 | 25 | 13 | 62 | 0.4 |
| Antibody . | Extranodal DLCL* . | Nodal DLCL* % of cases (n = 21) . | Total extranodal vs nodal DLCL† . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Testicular % of cases (n = 28) . | CNS % of cases (n = 11) . | |||||||||
| − . | +/− . | + . | − . | +/− . | + . | − . | +/− . | + . | P . | |
| HLA-A | 61 | 29 | 11 | 55 | 18 | 27 | 10 | 33 | 57 | 0.008 |
| β2M‡ | 23 | 46 | 31 | 18 | 27 | 55 | 11 | 5 | 84 | 0.7 |
| TAP‡ | 26 | 33 | 41 | 30 | 40 | 30 | 0 | 9 | 91 | 0.02 |
| HLA-DR | 61 | 29 | 11 | 46 | 18 | 36 | 5 | 10 | 85 | 0.004 |
| W6/32‡ | 62 | 0 | 38 | 75 | 0 | 25 | 25 | 13 | 62 | 0.4 |
HLA indicates human leukocyte antigen; DLCL, diffuse large B-cell lymphoma; CNS, central nervous system; β2M, β2-microglobulin.
The − indicates no detectable staining of tumor cells compared with staining of internal control cells (respectively, T lymphocytes, macrophages, and dendritic cells for HLA class I, β2M, and TAP as well as macrophages and dendritic cells for class II) within each tumor. The +/− indicates weaker or heterogeneous staining of tumor cells compared with internal control cells.
The P value was determined by using a two-sided Fisher exact test that compared the total loss of expression in extranodal (testis and CNS) and nodal DLCL.
Frozen tissue sections of 13 testicular, 4 CNS, and 8 nodal DLCLs were stained for W6/32. One testicular and one CNS lymphoma were not interpretable for TAP. Two testicular and 3 nodal lymphomas were not interpretable for β2M.