H7 and cycloheximide inhibit IL-6– and IFN-γ–induced fusion of macrophages with T-tropic envelope-expressing cells
| Cytokine* . | Inhibitor† . | No. of syncytia† . |
|---|---|---|
| None | None | 7 ± 4 |
| IL-6 | None | 72 ± 9 |
| IL-6 | Cx | 6 ± 2 |
| IL-6 | H7 | 9 ± 1 |
| None | Cx | 8 ± 4 |
| None | H7 | 3 ± 0 |
| None | None | 14 ± 1 |
| IFN-γ | None | 61 ± 13 |
| IFN-γ | Cx | 13 ± 1 |
| IFN-γ | H7 | 12 ± 2 |
| None | Cx | 11 ± 1 |
| None | H7 | 9 ± 1 |
| Cytokine* . | Inhibitor† . | No. of syncytia† . |
|---|---|---|
| None | None | 7 ± 4 |
| IL-6 | None | 72 ± 9 |
| IL-6 | Cx | 6 ± 2 |
| IL-6 | H7 | 9 ± 1 |
| None | Cx | 8 ± 4 |
| None | H7 | 3 ± 0 |
| None | None | 14 ± 1 |
| IFN-γ | None | 61 ± 13 |
| IFN-γ | Cx | 13 ± 1 |
| IFN-γ | H7 | 12 ± 2 |
| None | Cx | 11 ± 1 |
| None | H7 | 9 ± 1 |
IL-6 indicates interleukin 6; IFN-γ, interferon γ.
Cytokines were added for the last 24 hours of the monocyte-derived macrophage culture.
Cycloheximide (Cx) at 5 ng/mL and 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine (H7) at 100 μmol were added to MDMs 1 hour prior to cytokines.
Syncytia were scored after 18 hours of co-culture of the macrophages (untreated or pretreated with cytokines) and TF228 cells expressing IIIB envelope.