DCs were generated from FL-supplemented BALB/c BM cultures as described in “Materials and methods.” Cells were harvested after 9 days of culture in FL alone and then sorted for myeloid-type and lymphoid-type DCs with the use of CD11b and CD11c to delineate these populations (same gates as used in Figure 6B). Sorted DCs were then cultured in culture medium supplemented with GM-CSF, IFNγ, and SAC (Pansorbin) for 40 hours at a cell density of 1 × 10⁶cells per milliliter. Supernatants were assayed for murine IL-12 p70 by means of an enzyme-linked immunosorbent assay kit. Results are the average ± SD of 3 separate experiments.

IL indicates interleukin; DCs, dendritic cells.