Cultures were established with BM cells cultured in FL for 9 days or GM-CSF plus IL-4 for 7 days from C57BL/6 mice as described in “Materials and methods.” LPS, IFNα, or GM-CSF was added to FL-supplemented cultures on the eighth day of culture, and the cells were harvested 24 hours later for flow cytometric analysis. Numbers are the percentage positive, after subtraction of background with the use of the appropriate isotype controls as described in “Materials and methods.” Detection of the above antigens was performed by flow cytometric analysis a minimum of 3 times, with similar results obtained each time.

FL indicates flt3 ligand; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; DC; dendritic cell; LPS, lipopolysaccharide; IFN, interferon; (M), marker expression restricted to the MAC1^bright/CD11c^dullpopulation; (L), marker expression restricted to the MAC1^dull/CD11c^bright population; ND, not determined.

Values given as percentage positive.