Expression of MUC1 epitopes on multiple myeloma and healthy B-lineage cells in blood and bone marrow
| . | Percentage of cells expressing MUC1 epitopes . | |||
|---|---|---|---|---|
| B27.29 . | AR20.5 . | VU-4H5* . | VU-3C6* . | |
| MM patient cells | ||||
| MM plasma cells (BM) (5) | 67 ± 15† | 54 ± 20† | 55 ± 12† | 38 ± 13† |
| Range | 21-88 | 14-80 | 17-78 | 8-84 |
| MM B cells (PBMCs) (12) | 70 ± 6‡ | 75 ± 4‡ | 85 ± 3 | 57 ± 9 |
| Range | 36-93 | 57-88 | 58-95 | 15-98 |
| Healthy donor cells | ||||
| Plasma cells (BM) (3) | 13 ± 3 | 12 ± 9 | 6 ± 2 | 1 ± 0.4 |
| Range | 8-45 | 2-31 | 1-12 | 1-2 |
| B cells (PBMCs) (4) | 4 ± 1 | 1 ± 0.5 | 49 ± 10 | 64 ± 12 |
| Range | 1-8 | 1-2 | 25-74 | 33-90 |
| . | Percentage of cells expressing MUC1 epitopes . | |||
|---|---|---|---|---|
| B27.29 . | AR20.5 . | VU-4H5* . | VU-3C6* . | |
| MM patient cells | ||||
| MM plasma cells (BM) (5) | 67 ± 15† | 54 ± 20† | 55 ± 12† | 38 ± 13† |
| Range | 21-88 | 14-80 | 17-78 | 8-84 |
| MM B cells (PBMCs) (12) | 70 ± 6‡ | 75 ± 4‡ | 85 ± 3 | 57 ± 9 |
| Range | 36-93 | 57-88 | 58-95 | 15-98 |
| Healthy donor cells | ||||
| Plasma cells (BM) (3) | 13 ± 3 | 12 ± 9 | 6 ± 2 | 1 ± 0.4 |
| Range | 8-45 | 2-31 | 1-12 | 1-2 |
| B cells (PBMCs) (4) | 4 ± 1 | 1 ± 0.5 | 49 ± 10 | 64 ± 12 |
| Range | 1-8 | 1-2 | 25-74 | 33-90 |
MUC1 expression on BM plasma cells (CD38hi45RA−/lo scattermod/hi) and PB B cells (CD19+) was determined by flow cytometric analysis using 4 mAbs: B27.29, AR20.5, VU-4H5, and VU-3C6. Values reflect the percentage (mean ± standard error) of plasma cells or B cells that express the MUC1 epitope identified by these mAbs.
MM indicates multiple myeloma; BM, bone marrow; PBMCs, peripheral blood mononuclear cells.
As per Treon et al.26
P < .05 for comparison of MUC1 epitopes for MM patient plasma cells with HD plasma cells.
P < .05 for comparison of MM patient B cells with healthy donor B cells.