Vitamin D3-induced differentiation
| Subline . | CD14 (MFI) . | % in S + G2 + M . |
|---|---|---|
| pCIneo | ||
| C | 1 ± 0.1 | 33.4 ± 5.9 |
| D3 | 45 ± 11.8 | 7.6 ± 3.3 |
| Stat1HA.7 | ||
| C | 1 ± 0.01 | 40.1 ± 0.3 |
| D3 | 55 ± 19 | 7.1 ± 2.8 |
| Stat1Y701F.8 | ||
| C | 1 ± 0.1 | 32 ± 1.8 |
| D3 | 46 ± 10.6 | 4.1 ± 0.1 |
| Subline . | CD14 (MFI) . | % in S + G2 + M . |
|---|---|---|
| pCIneo | ||
| C | 1 ± 0.1 | 33.4 ± 5.9 |
| D3 | 45 ± 11.8 | 7.6 ± 3.3 |
| Stat1HA.7 | ||
| C | 1 ± 0.01 | 40.1 ± 0.3 |
| D3 | 55 ± 19 | 7.1 ± 2.8 |
| Stat1Y701F.8 | ||
| C | 1 ± 0.1 | 32 ± 1.8 |
| D3 | 46 ± 10.6 | 4.1 ± 0.1 |
U-937 sublines; pCIneo, Stat1HA.7, and Stat1Y701F.8 were induced by 1,25(OH)2 vitamin D3 for 72 hours. C indicates untreated control. Expression of the monocytic differentiation antigen CD14 mean fluorescence intensity (MFI, arbitrary units) was analyzed using flow cytometry. DNA content was measured by flow cytometry of propidium iodide–stained nuclei. Percent of the cells in S + G2 + M phase of the cell cycle was calculated using MacCycle Software. Data are presented as mean ± SD (n = 2).