MIP-1α values for T cells incubated with APCs
| T cells incubated with APCs . | MIP-1α, pg/mL . |
|---|---|
| T cells with allogeneic MIP-1α−/− B6 | |
| Line 150A | 195 ± 7.8 |
| Line 150B | 178 ± 11 |
| Line 150C | 245 ± 14 |
| T cells with syngeneic bm1 | |
| Line 150A | 7 ± 3.4 |
| Line 150B | 8.5 ± 2.8 |
| Line 150C | 5 ± 2.1 |
| T cells incubated with APCs . | MIP-1α, pg/mL . |
|---|---|
| T cells with allogeneic MIP-1α−/− B6 | |
| Line 150A | 195 ± 7.8 |
| Line 150B | 178 ± 11 |
| Line 150C | 245 ± 14 |
| T cells with syngeneic bm1 | |
| Line 150A | 7 ± 3.4 |
| Line 150B | 8.5 ± 2.8 |
| Line 150C | 5 ± 2.1 |
The MIP-1α values are given as the mean ± SE. T-cell lines were generated as indicated in the text. Bone marrow–derived macrophages (APCs) from MIP-1α−/− B6 and bm1 mice were incubated with the T-cell lines for 24 hours. The supernatants were then harvested, and the MIP-1α concentrations were determined using ELISA. The minimum level of detection of MIP-1α for this assay is 3.75 pg/mL. MIP-1α−/− B6 macrophages do not generate any detectable MIP-1α during the 24-hour incubation.