Quantitative assessment of GVHD in bm1 and bm12 recipients at day 6 (scale 0-4)
| . | GI tract . | Liver . | Lung . |
|---|---|---|---|
| bm1 | |||
| −/− | 1.3 ± 0.2 | 1.2 ± 0.1 | 1.3 ± 0.3 |
| +/+ | 1.2 ± 0.3 | 2.2 ± 0.33-150 | 1.3 ± 0.3 |
| bm12 | |||
| −/− | 1.3 ± 0.3 | 1.0 ± 0.5 | 2.5 ± 0 |
| +/+3-160 | 2 ± 0 | 1.0 ± 0 | 2.5 ± 0 |
| . | GI tract . | Liver . | Lung . |
|---|---|---|---|
| bm1 | |||
| −/− | 1.3 ± 0.2 | 1.2 ± 0.1 | 1.3 ± 0.3 |
| +/+ | 1.2 ± 0.3 | 2.2 ± 0.33-150 | 1.3 ± 0.3 |
| bm12 | |||
| −/− | 1.3 ± 0.3 | 1.0 ± 0.5 | 2.5 ± 0 |
| +/+3-160 | 2 ± 0 | 1.0 ± 0 | 2.5 ± 0 |
The quantitative assessment of GVHD was performed by one of us (A.P.-M.) blinded to the transplantation performed. The tissues were evaluated after transfer of MIP-1α−/− or MIP-1α+/+ splenocytes to 4-5 bm1 mice recipients in each instance except for the lung, in which only 2 bm1 recipients could be evaluated after transfer of wild type and MIP-1α−−splenocytes. Bile duct occlusion was found in 3 bm1 recipients after transfer of MIP-1α+/+ splenocytes. This was not observed in any bm1 recipient after the transfer of MIP-1α−/−splenocytes. The scale for the scoring of GVHD was established so that maximum inflammation would be given a grade of 4. The values presented are the mean ± SE for each group.
P = .03.
P = .116.