Clonal rearrangements obtained from 3 cases of Hodgkin disease
| Case no. . | Cells . | . | V gene3-150 . | Functionality3-151 . | Mutation frequency (%) . | Remarks . |
|---|---|---|---|---|---|---|
| 1 | HRS | 7 × VH3-20 | + | 2.7 | ||
| 3 × VH3-11 | ? | 6.2 | Deletion beginning in CDRIII and ending in the JH4/5 intron | |||
| 4 × VκO18/O8 | + | 1.2 | ||||
| 15 × VκA17 | − | 0 | ||||
| 11 × Vλ1g | − | 3.5 | ||||
| 13 × Vλ3r | + | 5.9 | 11 × identical, 2 × with nonsense mutation | |||
| SMALL | C1 | 2 × VH3-23 | + | 11.2 and 8.8 | ||
| CD30−/ | C1 | 2 × VκA19/A3 | + | 0 | ||
| EBER+ | C1 | 1 × Vλ1g | − | 9.8 | 1 bp del | |
| C1 | 1 × Vλ1c | + | 3.9 | |||
| C2 | 2 × VH3-20 | + | 2.6 and 6.3 | |||
| C2 | 1 × VκB3 | + | 8.1 | 1 stop, 1 bp ins | ||
| 2 | HRS | 6 × VH4-31 | + | 9.7 | ||
| 11 × VH4-34 | − | 14 | 11 bp del FRII, large FRIII and CDRIII del | |||
| 6 × VκO14 | − | 0 | ||||
| 6 × Vλ1v117(1b) | − | 17 | 5 bp ins, 14 bp dup | |||
| 11 × Vλ3m | + | 12.8 | ||||
| SMALL | C1 | 2 × VH4-04 | + | 3.7 | ||
| CD30−/ | C2 | 2 × VH4-34 | + | 8.6 | ||
| EBER+ | C2 | 1 × VκA27 | + | 5 | ||
| C2 | 1 × Vλ3f | − | 0 | |||
| C3 | 1 × VκO18/O8 | − | 0 | |||
| C3 | 3 × Vλ1v117(1b) | + | 1.5 | |||
| 3 | HRS | 2 × VκA19/A3 | − | 9.5 | ||
| 8 × VκB3 | − | 0 | ||||
| 9 × Vλ6a | + | 11 | 3 stops (1 seq with 1 nt difference) | |||
| SMALL | C1 | 2 × Vκ014/04 | − | 0 | ||
| CD30−/ | ||||||
| EBER+ |
| Case no. . | Cells . | . | V gene3-150 . | Functionality3-151 . | Mutation frequency (%) . | Remarks . |
|---|---|---|---|---|---|---|
| 1 | HRS | 7 × VH3-20 | + | 2.7 | ||
| 3 × VH3-11 | ? | 6.2 | Deletion beginning in CDRIII and ending in the JH4/5 intron | |||
| 4 × VκO18/O8 | + | 1.2 | ||||
| 15 × VκA17 | − | 0 | ||||
| 11 × Vλ1g | − | 3.5 | ||||
| 13 × Vλ3r | + | 5.9 | 11 × identical, 2 × with nonsense mutation | |||
| SMALL | C1 | 2 × VH3-23 | + | 11.2 and 8.8 | ||
| CD30−/ | C1 | 2 × VκA19/A3 | + | 0 | ||
| EBER+ | C1 | 1 × Vλ1g | − | 9.8 | 1 bp del | |
| C1 | 1 × Vλ1c | + | 3.9 | |||
| C2 | 2 × VH3-20 | + | 2.6 and 6.3 | |||
| C2 | 1 × VκB3 | + | 8.1 | 1 stop, 1 bp ins | ||
| 2 | HRS | 6 × VH4-31 | + | 9.7 | ||
| 11 × VH4-34 | − | 14 | 11 bp del FRII, large FRIII and CDRIII del | |||
| 6 × VκO14 | − | 0 | ||||
| 6 × Vλ1v117(1b) | − | 17 | 5 bp ins, 14 bp dup | |||
| 11 × Vλ3m | + | 12.8 | ||||
| SMALL | C1 | 2 × VH4-04 | + | 3.7 | ||
| CD30−/ | C2 | 2 × VH4-34 | + | 8.6 | ||
| EBER+ | C2 | 1 × VκA27 | + | 5 | ||
| C2 | 1 × Vλ3f | − | 0 | |||
| C3 | 1 × VκO18/O8 | − | 0 | |||
| C3 | 3 × Vλ1v117(1b) | + | 1.5 | |||
| 3 | HRS | 2 × VκA19/A3 | − | 9.5 | ||
| 8 × VκB3 | − | 0 | ||||
| 9 × Vλ6a | + | 11 | 3 stops (1 seq with 1 nt difference) | |||
| SMALL | C1 | 2 × Vκ014/04 | − | 0 | ||
| CD30−/ | ||||||
| EBER+ |
bp indicates base pair; del, deletion; ins, insertion; dup, duplication; FR, framework region; CDR, complementarity determining region; C1, C2, C3, clone 1, clone 2, clone 3.
Given is the number of cells from which a given rearrangement was amplified and the name of the V gene segment used in the rearrangement.
Functionality of the rearrangement before onset of somatic hypermutation.