Oligonucleotides (5′ → 3′) used for generation of UTY minigenes
| position UTY gene (bp) . | Sequence (5′ → 3′) . |
|---|---|
| 79-120 | For: AGCTTCACCATGGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCTAG Rev: GATCCTAGACTGTCAGGCTAACAGACTCCTCTTCACTCTCGCGGCTCGCCATGGTGA |
| 172-216 | For: AGCTTCACCATGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTAGGCAAGTAG Rev: GATCCTACTTGCCTAGTAGGGTCTTCGTTCTGGCGCCATCTTCATGAAGCCTCATGGTGA |
| 484-522 | For: AGCTTCACCATGCGAGCCAAGGAAATTCATTTACGACTTGGGCTCATGTTCTAG Rev: GATCCTAGAACATGAGCCCAAGTCGTAAATGAATTTCCTTGGCTCGCATGGTGA |
| position UTY gene (bp) . | Sequence (5′ → 3′) . |
|---|---|
| 79-120 | For: AGCTTCACCATGGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCTAG Rev: GATCCTAGACTGTCAGGCTAACAGACTCCTCTTCACTCTCGCGGCTCGCCATGGTGA |
| 172-216 | For: AGCTTCACCATGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTAGGCAAGTAG Rev: GATCCTACTTGCCTAGTAGGGTCTTCGTTCTGGCGCCATCTTCATGAAGCCTCATGGTGA |
| 484-522 | For: AGCTTCACCATGCGAGCCAAGGAAATTCATTTACGACTTGGGCTCATGTTCTAG Rev: GATCCTAGAACATGAGCCCAAGTCGTAAATGAATTTCCTTGGCTCGCATGGTGA |