Nomenclature of plasmids and their characteristics
| Plasmid . | Promoter . | 3′ Enhancer . |
|---|---|---|
| pWT | Wild-type | — |
| pWT-WT | Wild-type | Wild-type |
| pWT-del DR-2 | Wild-type | DR-2 deleted |
| pWT-half DR-2 | Wild-type | Half DR-2 |
| pm123 | Mutant | — |
| pm123-WT | Mutant | Wild-type |
| pWT-mut HIF | Wild-type | Mutated HIF-1 site |
| pWT-mut CATGG | Wild-type | Mutated CATGG site |
| Plasmid . | Promoter . | 3′ Enhancer . |
|---|---|---|
| pWT | Wild-type | — |
| pWT-WT | Wild-type | Wild-type |
| pWT-del DR-2 | Wild-type | DR-2 deleted |
| pWT-half DR-2 | Wild-type | Half DR-2 |
| pm123 | Mutant | — |
| pm123-WT | Mutant | Wild-type |
| pWT-mut HIF | Wild-type | Mutated HIF-1 site |
| pWT-mut CATGG | Wild-type | Mutated CATGG site |
Mutations in the mouse Epo promoter and 3′ enhancer are shown in Figure 3A. The mouse Epo promoter (144 bp from −122 to +22) and 3′ enhancer (51 bp from +3838 to +3888) were ligated upstream and downstream of the luciferase gene, respectively (mP and mE in the top of Figure 3B).