In vivo effect of Ets-site mutations in theFlk-1 promoter
| Mutated site . | Wild-type sequence . | Mutant sequence . | TG . | EC . |
|---|---|---|---|---|
| wt | wt | wt | 15 | 6 |
| E#3 | TTCCGG | TTggGG | 14 | 4 |
| E#6 | CCGGAC | CCccAC | 13 | 7 |
| Mutated site . | Wild-type sequence . | Mutant sequence . | TG . | EC . |
|---|---|---|---|---|
| wt | wt | wt | 15 | 6 |
| E#3 | TTCCGG | TTggGG | 14 | 4 |
| E#6 | CCGGAC | CCccAC | 13 | 7 |
The number of embryos is indicated. Embryos were transgenic for LacZ reporter gene constructs containing the 939-bp Flk-1promoter with mutations in the indicated Ets sites and the 430-bp minimal enhancer. Abbreviations are shown in Table 2. The position of the Ets sites in the Flk-1 promoter is depicted in Figure 6.