Name and sequence of primers used
| . | Forward primers . | Reverse primers . |
|---|---|---|
| SSCP analysis | EX3S: | EX3AS: |
| 5′gCCTgTCCTCCCACCACCCTCTC3′ | 5′AgCTgCTTgCTgAAgATCCg3′ | |
| EX4S: | EX4AS: | |
| 5′gTgggTTTgTTgCCATgAAACg3′ | 5′CARCCCTgATgTCTgCATTTgTCC3′ | |
| EX5S: | EX5AS: | |
| 5′CCCAAggAATCTgAgACATggTCC3′ | 5′TgTTCAggCCACCAACCTCATTC3′ | |
| LOH detection on 21q | D21S369S: | D21S369AS: |
| 5′ATgAggAggTATTCAATTATggTA3′ | 5′gCTgATATggTAAgTACATTTATC3′ | |
| D21S258 S: | D21S258 AS: | |
| 5′CTCAAATACgACTCCAgAgg3′ | 5′ggACAgTgTTCACTATTCAg3′ | |
| D21S65 S: | D21S65 AS: | |
| 5′CCgAAAACTTACTggAgAAC3′ | 5′gATCATCCAggAATCACCAA3′ | |
| D21S270 S: | D21S270 AS: | |
| 5′gAAATgTTTTAATAAATggTggTTA3′ | 5′ACAAAAgTTATggTCAAgggg3′ | |
| D21S49 S: | D21S49 AS: | |
| 5′CTCCCCCgAggTTCCACT3′ | 5′ATCAAATCCTAAAgCCgACTgTCT3′ | |
| D21S1259 S: | D21S1259 AS: | |
| 5′gggACTgTAATAAATATTCTgTTgg3′ | 5′CACTggCTCTCCTgACC3′ |
| . | Forward primers . | Reverse primers . |
|---|---|---|
| SSCP analysis | EX3S: | EX3AS: |
| 5′gCCTgTCCTCCCACCACCCTCTC3′ | 5′AgCTgCTTgCTgAAgATCCg3′ | |
| EX4S: | EX4AS: | |
| 5′gTgggTTTgTTgCCATgAAACg3′ | 5′CARCCCTgATgTCTgCATTTgTCC3′ | |
| EX5S: | EX5AS: | |
| 5′CCCAAggAATCTgAgACATggTCC3′ | 5′TgTTCAggCCACCAACCTCATTC3′ | |
| LOH detection on 21q | D21S369S: | D21S369AS: |
| 5′ATgAggAggTATTCAATTATggTA3′ | 5′gCTgATATggTAAgTACATTTATC3′ | |
| D21S258 S: | D21S258 AS: | |
| 5′CTCAAATACgACTCCAgAgg3′ | 5′ggACAgTgTTCACTATTCAg3′ | |
| D21S65 S: | D21S65 AS: | |
| 5′CCgAAAACTTACTggAgAAC3′ | 5′gATCATCCAggAATCACCAA3′ | |
| D21S270 S: | D21S270 AS: | |
| 5′gAAATgTTTTAATAAATggTggTTA3′ | 5′ACAAAAgTTATggTCAAgggg3′ | |
| D21S49 S: | D21S49 AS: | |
| 5′CTCCCCCgAggTTCCACT3′ | 5′ATCAAATCCTAAAgCCgACTgTCT3′ | |
| D21S1259 S: | D21S1259 AS: | |
| 5′gggACTgTAATAAATATTCTgTTgg3′ | 5′CACTggCTCTCCTgACC3′ |
SSCP indicates single-stranded conformation polymorphism; LOH, loss of heterozygosity.