Table 1. Sequence of the primers used to... | American Society of Hematology

Table 1.

Sequence of the primers used to amplify exons, exon/intron junctions, and presumed regulatory region of the SH201A

	Forward primer	Reverse primer	bp	Temp (°C)
XLP.1	cacgacgttgtaaaacgacacagaagcattactaagc	ggataacaatttcacacaggccagctgctgttgcccac	608	55
XLP.2	cacgacgttgtaaaacgaccaatgacaccatatacgtgt	ggataacaatttcacacagggctttcttaatgatccatga	380	55
XLP.3	cacgacgttgtaaaacgaccaagttacacaaatgtta	ggataacaatttcacacaggcttggactcataactctg	348	55
XLP.4	cacgacgttgtaaaacgactcattgtgagttttatgcag	ggataacaatttcacacagggctcaccgaactgtatta	272	55
XLP.A	cacgacgttgtaaaacgacaaagcaccagttaaatgattc	ggataacaatttcacacaggtcacacttcagttaaaacgag	718	55

	Forward primer	Reverse primer	bp	Temp (°C)
XLP.1	cacgacgttgtaaaacgacacagaagcattactaagc	ggataacaatttcacacaggccagctgctgttgcccac	608	55
XLP.2	cacgacgttgtaaaacgaccaatgacaccatatacgtgt	ggataacaatttcacacagggctttcttaatgatccatga	380	55
XLP.3	cacgacgttgtaaaacgaccaagttacacaaatgtta	ggataacaatttcacacaggcttggactcataactctg	348	55
XLP.4	cacgacgttgtaaaacgactcattgtgagttttatgcag	ggataacaatttcacacagggctcaccgaactgtatta	272	55
XLP.A	cacgacgttgtaaaacgacaaagcaccagttaaatgattc	ggataacaatttcacacaggtcacacttcagttaaaacgag	718	55

The intronic primers were tagged at the 5′ end by oligos complementary to M13 forward and reverse primers (italics) to be able to sequence the amplified products using IR 800 M13 forward and reverse primers from Li-Cor.

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