Flow cytometric analysis of lymphocytes and CD34+ cells for cell-surface binding of annexin V 24 hours after UV-B
| J/m2 . | CD34+ cell fraction . | Whole MNC . | ||
|---|---|---|---|---|
| Viable cells (%) . | Annexin V+ cells (%) . | Viable cells (%) . | Annexin V+ cells (%) . | |
| (Annexin V−/PI−) . | . | (Annexin V−/PI−) . | . | |
| Pre | 94.4 | 5.6 | 73.7 | 21.7 |
| 0 | 82.6 | 17.4 | 65.0 | 25.0 |
| 33 | 78.6 | 18.7 | 16.2 | 69.8 |
| 66 | 79.2 | 20.0 | 10.2 | 81.6 |
| 100 | 75.8 | 24.2 | 7.1 | 79.5 |
| J/m2 . | CD34+ cell fraction . | Whole MNC . | ||
|---|---|---|---|---|
| Viable cells (%) . | Annexin V+ cells (%) . | Viable cells (%) . | Annexin V+ cells (%) . | |
| (Annexin V−/PI−) . | . | (Annexin V−/PI−) . | . | |
| Pre | 94.4 | 5.6 | 73.7 | 21.7 |
| 0 | 82.6 | 17.4 | 65.0 | 25.0 |
| 33 | 78.6 | 18.7 | 16.2 | 69.8 |
| 66 | 79.2 | 20.0 | 10.2 | 81.6 |
| 100 | 75.8 | 24.2 | 7.1 | 79.5 |
Results shown are representative of 3 different experiments using cord blood. Similar results were obtained when MNC from PBSC were irradiated.
Numbers of whole MNC and CD34+ cells evaluated were 4000 and 600, respectively.