Digestion of IL-8 by gelatinase B and aminoterminal sequence analysis
| . | Aminoterminal sequences . | Before digestion (%)* . | After digestion (%)* . |
|---|---|---|---|
| Natural IL-8 | |||
| IL-8(-2-77) | EGAVLPR … | 10 | 0 |
| IL-8(1-77) | AVLPRSA … | 35 | 0 |
| IL-8(6-77) | SAKELRCQC … | 55 | 60 |
| IL-8(7-77) | AKELRCQC … | 0 | 40 |
| Recombinant IL-8 | |||
| IL-8(1-77) | AVLPRSA … | 100 | 0 |
| IL-8(7-77) | AKELRCQC … | 0 | 100 |
| . | Aminoterminal sequences . | Before digestion (%)* . | After digestion (%)* . |
|---|---|---|---|
| Natural IL-8 | |||
| IL-8(-2-77) | EGAVLPR … | 10 | 0 |
| IL-8(1-77) | AVLPRSA … | 35 | 0 |
| IL-8(6-77) | SAKELRCQC … | 55 | 60 |
| IL-8(7-77) | AKELRCQC … | 0 | 40 |
| Recombinant IL-8 | |||
| IL-8(1-77) | AVLPRSA … | 100 | 0 |
| IL-8(7-77) | AKELRCQC … | 0 | 100 |
Relative amounts were determined by aminoterminal sequence analysis.