PU.1 blocks erythroid cell differentiation
| Cell line . | With/without induction . | Benzidine-positive cells (%) . |
|---|---|---|
| ZnSO4 | ||
| K562/pC18 (vector)* | − | 35.0 |
| + | 32.5 | |
| K562/mPU.1 no. 3* | − | 46.4 |
| + | 6.7 | |
| K562/mPU.1 no. 7* | − | 35.0 |
| + | 8.9 | |
| β-Estradiol induction | ||
| G1ER/M† | + | 52.0 |
| − | 0.5 | |
| G1ER/mPU.1 no. 1† | + | 6.8 |
| − | 0.5 | |
| G1ER/mPU.1 no. 2† | + | 5.6 |
| − | 0.5 | |
| Cell line . | With/without induction . | Benzidine-positive cells (%) . |
|---|---|---|
| ZnSO4 | ||
| K562/pC18 (vector)* | − | 35.0 |
| + | 32.5 | |
| K562/mPU.1 no. 3* | − | 46.4 |
| + | 6.7 | |
| K562/mPU.1 no. 7* | − | 35.0 |
| + | 8.9 | |
| β-Estradiol induction | ||
| G1ER/M† | + | 52.0 |
| − | 0.5 | |
| G1ER/mPU.1 no. 1† | + | 6.8 |
| − | 0.5 | |
| G1ER/mPU.1 no. 2† | + | 5.6 |
| − | 0.5 | |
PU.1 blocks K562 cell differentiation, as assessed by benzidine-positive cells as an indicator. K562/pC18: K562 stably transfected with empty metallothionein promoter vector. K562/PU.1 no. 3 and no. 7: K562 stably transfected with metallothionein-promoter-driven mouse PU.1 expression vector clone no. 3 and no. 7, respectively. The cells were induced with hemin to differentiate to mature red blood cells. 100 μmol/L ZnSO4 was used to induce PU.1 expression.
PU.1 blocks G1ER cell differentiation. G1ER/M: G1ER cells infected with an empty virus. G1ER/mPU.1 no. 1: clone no. 1 of PU.1-virus–infected G1ER cells. G1ER/mPU.1 no. 2: clone no. 2 of PU.1-virus–infected G1ER cells. 3.3 × 10−8mol/L β-estradiol was used to induce functional GATA-1 activity.