Primer pairs, annealing temperatures, and detection methods used in gene assays based on polymerase chain reaction
| Gene . | Primers . | Annealing temperature (°C) . | Detection method . |
|---|---|---|---|
| IL6 (G-174C) | F: TTG TCA AGA CAT GCC AAG TGC | 67 | NlaIII digest, 3% agarose SFR |
| R: CAG AAT GAG CCT CAG AGA CAT CTC C | |||
| TNF (G-308A) | F: CAA AAG AAA TGG AGG CAA TAG GTT TTG AGG GCC AT | 63 | NcoI digest, 3% agarose SFR |
| R: AGG GCG GGG AAA GAA TCA TTC AAC CAG CGG AAA AC | |||
| LTA (NcoI, G to A) | F: CCG TGC TTC GTG CTT TGG ACT A | 64 | NcoI digest, 2% agarose |
| R: AGA GCT GGT GGG GAC ATG TCT G | |||
| IL1A (G-889T) | F: GGG GGC TTC ACT ATG TTG CCC ACA CTG GAC TAA | 58 | NcoI digest, 2% agarose SFR |
| R: GAA GGC ATG GAT TTT TAC ATA TGA CCT TCC ATG | |||
| IL1B (C + 3953T) | F: CTC AGG TGT CCT CGA AGA AAT CAA A | 58 | TaqI digest, 2% agarose |
| R: GCT TTT TTG CTG TGA GTC CCG | |||
| IL1RN (intron 2 VNTR) | F: CTC AGC AAC ACT CCT AT | 58 | Size fractionation, 2% TreviGel 500 (Trevigen, Gaithersburg, MD) |
| R: TCC TGG TCT GCA GGT AA | |||
| SDF1 (G801A) | F: GGT GCC AGG ACC AGT CAA C | 55 | MspI digest, 2% agarose |
| R: AGC TTT GGT GCT GAG AGT CC | |||
| CCR5 (Δ32) | F: CTT CAT TAC ACC TGC AGC TC | 58 | Size fractionation, 3% agarose SFR |
| R: CAG CCC TGT GCC TCT TCT TC |
| Gene . | Primers . | Annealing temperature (°C) . | Detection method . |
|---|---|---|---|
| IL6 (G-174C) | F: TTG TCA AGA CAT GCC AAG TGC | 67 | NlaIII digest, 3% agarose SFR |
| R: CAG AAT GAG CCT CAG AGA CAT CTC C | |||
| TNF (G-308A) | F: CAA AAG AAA TGG AGG CAA TAG GTT TTG AGG GCC AT | 63 | NcoI digest, 3% agarose SFR |
| R: AGG GCG GGG AAA GAA TCA TTC AAC CAG CGG AAA AC | |||
| LTA (NcoI, G to A) | F: CCG TGC TTC GTG CTT TGG ACT A | 64 | NcoI digest, 2% agarose |
| R: AGA GCT GGT GGG GAC ATG TCT G | |||
| IL1A (G-889T) | F: GGG GGC TTC ACT ATG TTG CCC ACA CTG GAC TAA | 58 | NcoI digest, 2% agarose SFR |
| R: GAA GGC ATG GAT TTT TAC ATA TGA CCT TCC ATG | |||
| IL1B (C + 3953T) | F: CTC AGG TGT CCT CGA AGA AAT CAA A | 58 | TaqI digest, 2% agarose |
| R: GCT TTT TTG CTG TGA GTC CCG | |||
| IL1RN (intron 2 VNTR) | F: CTC AGC AAC ACT CCT AT | 58 | Size fractionation, 2% TreviGel 500 (Trevigen, Gaithersburg, MD) |
| R: TCC TGG TCT GCA GGT AA | |||
| SDF1 (G801A) | F: GGT GCC AGG ACC AGT CAA C | 55 | MspI digest, 2% agarose |
| R: AGC TTT GGT GCT GAG AGT CC | |||
| CCR5 (Δ32) | F: CTT CAT TAC ACC TGC AGC TC | 58 | Size fractionation, 3% agarose SFR |
| R: CAG CCC TGT GCC TCT TCT TC |
F indicates forward; R, reverse; and SFR, super fine resolution (Amresco, Solon, OH).