BCR/ABL p190 del C transgenic founders
| Founder no. . | Age at death (mo) . | RT-PCR* . | Cause of death . | Progeny . | Est. transgene copy number . |
|---|---|---|---|---|---|
| 4208 | 28 | + | Lymphoma | Yes | 3 |
| 4212 | 24 | − | Unknown† | No | 1 |
| 4227 | 21 | + | Unknown† | No | 6-8 |
| 4231 | 21 | − | Infection‡ | Yes | 3 |
| 4242 | 22 | + | Unknown† | No | 2 |
| 4243 | 27.5 | + | Carcinoid lung tumor1-153 | Yes | 3 |
| 4237 | 27.5 | + | Lung adenoma | Yes | 2 |
| 4266 | 12 | − | Found dead | Yes | 1 |
| Founder no. . | Age at death (mo) . | RT-PCR* . | Cause of death . | Progeny . | Est. transgene copy number . |
|---|---|---|---|---|---|
| 4208 | 28 | + | Lymphoma | Yes | 3 |
| 4212 | 24 | − | Unknown† | No | 1 |
| 4227 | 21 | + | Unknown† | No | 6-8 |
| 4231 | 21 | − | Infection‡ | Yes | 3 |
| 4242 | 22 | + | Unknown† | No | 2 |
| 4243 | 27.5 | + | Carcinoid lung tumor1-153 | Yes | 3 |
| 4237 | 27.5 | + | Lung adenoma | Yes | 2 |
| 4266 | 12 | − | Found dead | Yes | 1 |
Expression was measured at 4 weeks of age on peripheral blood samples using RT-PCR and hybridization with a specific oligonucleotide probe as described previously for BCR/ABL p190 transgenics.22
No significant pathologic findings were noted.
Animal had splenomegaly with a left shift in the myeloid series and decreased B- and T-cell numbers in bone marrow. However, no Bcr/Abl or endogenous c-Abl expression could be detected in the spleen.
Bcr/Abl protein could not be detected in the tumor. The animal also had regenerative liver nodules/hyperplasia.