Effect of fucoidan treatment on blood cell counts
| . | WBC (per μL) . | Neutrophils (per μL) . | Lymphocytes (per μL) . | Monocytes (per μL) . | Eosinophils (per μL) . | Platelets (×103/μL) . |
|---|---|---|---|---|---|---|
| WT PBS | 3 540 ± 256 | 1 022 ± 184 | 2 374 ± 218 | 63 ± 12 | 74 ± 17 | 920 ± 47 |
| WT FUC | 8 020 ± 1094* | 3 078 ± 617* | 4 816 ± 567* | 65 ± 30 | 101 ± 71 | 533 ± 132* |
| E−/− PBS | 3 120 ± 503 | 720 ± 144 | 2 328 ± 398 | 45 ± 15 | 34 ± 17 | 1 019 ± 26 |
| E−/− FUC | 23 333 ± 1 466* | 9 373 ± 901* | 13 695 ± 1 196* | 230 ± 86* | 33 ± 33 | 558 ± 44* |
| P/E−/− PBS | 9 125 ± 1 916 | 5 248 ± 1 239 | 3 423 ± 891 | 132 ± 50 | 71 ± 28 | 966 ± 66 |
| P/E−/− FUC | 26 500 ± 5 804* | 13 140 ± 4 870 | 12 355 ± 1 827* | 704 ± 207* | 177 ± 106 | 561 ± 66* |
| P/E−/− (spl) PBS | 23 016 ± 5 258 | 11 722 ± 2 692 | 12 353 ± 2 830 | 376 ± 55 | 244 ± 211 | 1312 ± 81 |
| P/E−/− (spl) FUC | 40 550 ± 8 584 | 22 119 ± 4 684 | 19 065 ± 3 417 | 485 ± 191 | 216 ± 137 | 954 ± 160 |
| . | WBC (per μL) . | Neutrophils (per μL) . | Lymphocytes (per μL) . | Monocytes (per μL) . | Eosinophils (per μL) . | Platelets (×103/μL) . |
|---|---|---|---|---|---|---|
| WT PBS | 3 540 ± 256 | 1 022 ± 184 | 2 374 ± 218 | 63 ± 12 | 74 ± 17 | 920 ± 47 |
| WT FUC | 8 020 ± 1094* | 3 078 ± 617* | 4 816 ± 567* | 65 ± 30 | 101 ± 71 | 533 ± 132* |
| E−/− PBS | 3 120 ± 503 | 720 ± 144 | 2 328 ± 398 | 45 ± 15 | 34 ± 17 | 1 019 ± 26 |
| E−/− FUC | 23 333 ± 1 466* | 9 373 ± 901* | 13 695 ± 1 196* | 230 ± 86* | 33 ± 33 | 558 ± 44* |
| P/E−/− PBS | 9 125 ± 1 916 | 5 248 ± 1 239 | 3 423 ± 891 | 132 ± 50 | 71 ± 28 | 966 ± 66 |
| P/E−/− FUC | 26 500 ± 5 804* | 13 140 ± 4 870 | 12 355 ± 1 827* | 704 ± 207* | 177 ± 106 | 561 ± 66* |
| P/E−/− (spl) PBS | 23 016 ± 5 258 | 11 722 ± 2 692 | 12 353 ± 2 830 | 376 ± 55 | 244 ± 211 | 1312 ± 81 |
| P/E−/− (spl) FUC | 40 550 ± 8 584 | 22 119 ± 4 684 | 19 065 ± 3 417 | 485 ± 191 | 216 ± 137 | 954 ± 160 |
Mice were treated with 2 doses of fucoidan (FUC; 25 mg/kg) or vehicle (phosphate-buffered saline [PBS]) over 6 hours. Leukocyte and platelet counts were assessed using an automated cell counter and leukocyte subsets were determined using Wright-stained smears.
WT, wild-type; E−/−, E-selectin-deficient; P/E−/−, P- and E-selectin-deficient; spl, splenectomized.
P < .05 compared with PBS, n = 3-10.