In vivo transformation of Stat5a/b-deficient cells bybcr-abl transforming genes
| BM cells . | Gene . | Myeloid B cell . | Mixed . | NT* . | |
|---|---|---|---|---|---|
| Wild-type | bcr-ablp210 | 8 | 0 | 1 | 6 |
| Stat5a/b(−/−) | bcr-ablp210 | 2 | 4 | 5 | 4 |
| Wild-type | bcr-ablp185 | 2 | 4 | 2 | 0 |
| Stat5a/b(−/−) | bcr-ablp185 | 2 | 4 | 0 | 2 |
| BM cells . | Gene . | Myeloid B cell . | Mixed . | NT* . | |
|---|---|---|---|---|---|
| Wild-type | bcr-ablp210 | 8 | 0 | 1 | 6 |
| Stat5a/b(−/−) | bcr-ablp210 | 2 | 4 | 5 | 4 |
| Wild-type | bcr-ablp185 | 2 | 4 | 2 | 0 |
| Stat5a/b(−/−) | bcr-ablp185 | 2 | 4 | 0 | 2 |
In vivo transformation: Lethally irradiated mice were reconstituted with bone marrow from Stat5-deficient and control heterozygous animals that has been cocultivated with GP + E86 producer cells for MSCV-bcr-abl p210/IRES-GFP and MSCV-bcr-abl p185/IRES-GFP for 48 hours. Sick animals were killed and the peripheral blood, as well as histologic sections, were analyzed for the presence of leukemic cells. The cell lineages involved in the tumors were characterized based on FACS analysis of peripheral blood samples.
BM indicates bone marrow.
Not determined.