In vitro cytokine-stimulated growth of CD34+CD38+ and CD34+CD38− cells
| Patient . | CD34+CD38+cells . | CD34+CD38− cells . | ||||
|---|---|---|---|---|---|---|
| Fold expansion (% of normal) . | % blasts (normal) . | % 5q− . | Fold expansion (% of normal) . | % blasts (normal) . | % 5q− . | |
| 4 | 268 (25) | 2 (1) | 91 | 15 (23) | 42 (73) | 96 |
| 7 | 377 (38) | 7 (nd) | 42 | 42 (6) | 30 (nd) | 97 |
| 8 | 181 (18) | 3 (nd) | 67 | 7 (1) | 18 (35) | 69 |
| 10 | 17 (nd) | 0 (nd) | 73 | 9 (4) | 89 (74) | 99 |
| 12 | 202 (31) | 16 (3) | 98 | 522 (124) | 11 (nd) | 99 |
| Patient . | CD34+CD38+cells . | CD34+CD38− cells . | ||||
|---|---|---|---|---|---|---|
| Fold expansion (% of normal) . | % blasts (normal) . | % 5q− . | Fold expansion (% of normal) . | % blasts (normal) . | % 5q− . | |
| 4 | 268 (25) | 2 (1) | 91 | 15 (23) | 42 (73) | 96 |
| 7 | 377 (38) | 7 (nd) | 42 | 42 (6) | 30 (nd) | 97 |
| 8 | 181 (18) | 3 (nd) | 67 | 7 (1) | 18 (35) | 69 |
| 10 | 17 (nd) | 0 (nd) | 73 | 9 (4) | 89 (74) | 99 |
| 12 | 202 (31) | 16 (3) | 98 | 522 (124) | 11 (nd) | 99 |
CD34+CD38+ and CD34+CD38− cells were cultured in FCS-containing medium in the presence of SCF, FL, MGDF, IL-3, G-CSF, GM-CSF (all at 25 ng/mL), and Epo (5 U/mL). After 10 to 14 days of incubation, cellular expansion was established and cytospin preparations were made for morphologic and FISH analysis. Purified CD34+CD38+ and CD34+CD38− cells from normal volunteers were investigated in parallel in all experiments. FISH analysis of normal cells showed a mean of 1% (n = 2) 1O + 2G cells for CD34+CD38− and 1.3% (n = 3) for CD34+CD38+ cells. Expansion of CD34+CD38+ and CD34+CD38− cells from normal donors varied from 650-fold to 1085-fold and from 64-fold to 755-fold, respectively.
nd indicates not determined.