Table 1. Phenotype of DG75 wild-type,... | American Society of Hematology

Table 1.

Phenotype of DG75 wild-type, vector, hIL-10, and vIL-10 transfectants

	Wild-type	Vector	hIL-10 line	hIL-10 cl-17	hIL-10 cl-30	hIL-10 cl-6	vIL-10 line	vIL-10 cl-1	vIL-10 cl-9	vIL-10 cl-19
CD11a	5	6	3	2	10	3	3	8	4	4
CD40	34	32	35	47	36	40	32	40	29	50
CD54	19	52	42	40	16	33	13	7	25	19
CD80	41	47	54	75	20	53	25	11	16	35
CD86	10	17	15	16	17	14	11	11	18	17
HLA-ABC	296	246	290	148	496	401	213	426	72	401
HLA-DR	357	331	340	131	485	216	352	444	215	200
CD95 (Fas)	18	21	24	21	ND	23	27	ND	21	19
IL-10 (U/mL)^*	<0.05	<0.05	154 ± 30	678 ± 112	251 ± 54	68 ± 17	73 ± 12	658 ± 78	114 ± 24	726 ± 136

	Wild-type	Vector	hIL-10 line	hIL-10 cl-17	hIL-10 cl-30	hIL-10 cl-6	vIL-10 line	vIL-10 cl-1	vIL-10 cl-9	vIL-10 cl-19
CD11a	5	6	3	2	10	3	3	8	4	4
CD40	34	32	35	47	36	40	32	40	29	50
CD54	19	52	42	40	16	33	13	7	25	19
CD80	41	47	54	75	20	53	25	11	16	35
CD86	10	17	15	16	17	14	11	11	18	17
HLA-ABC	296	246	290	148	496	401	213	426	72	401
HLA-DR	357	331	340	131	485	216	352	444	215	200
CD95 (Fas)	18	21	24	21	ND	23	27	ND	21	19
IL-10 (U/mL)^*	<0.05	<0.05	154 ± 30	678 ± 112	251 ± 54	68 ± 17	73 ± 12	658 ± 78	114 ± 24	726 ± 136

Mean log fluorescence intensity as measured by FACS. Representative data from one of 5 independent experiments. ND: not determined.

Production of IL-10 by 10⁶ cells/mL per 36 hours, as measured by enzyme-linked immunosorbent assay (ELISA). Mean ± SEM of 4 independent experiments.