Effect of different growth conditions on HCD57 cells proliferation survival an signal transduction
| Growth condition . | EPO . | 10 μmol/L orthovanadate . | 70 μmol/L orthovanadate . | SCF . | |
|---|---|---|---|---|---|
| Physiologic effect on HCD57 cells | Protection from apoptosis | +++ | − | +++ | − |
| Proliferation | +++ | +/− | − | + | |
| Activation of signal transduction molecules | EPOR | +++ | − | + | |
| JAK2 | +++ | − | +++ | − | |
| JAK1 | − | − | − | − | |
| Tyk2 | − | − | − | nd | |
| Vav | − | − | − | nd | |
| STAT1 | +++ | − | +++ | − | |
| STAT3 | − | − | − | − | |
| STAT5 | +++ | − | ++ | − | |
| SHC | +++ | − | − | +++ | |
| ERK-1 | +++ | +/− | +/− | +++ | |
| JNK | +++ | ++++ | +++++ | +++ | |
| P38 | +++ | − | +++ | nd | |
| PKB/Akt | +++ | − | +++ | ++ | |
| BAD | +++ | − | ++++ | +/− | |
| Growth condition . | EPO . | 10 μmol/L orthovanadate . | 70 μmol/L orthovanadate . | SCF . | |
|---|---|---|---|---|---|
| Physiologic effect on HCD57 cells | Protection from apoptosis | +++ | − | +++ | − |
| Proliferation | +++ | +/− | − | + | |
| Activation of signal transduction molecules | EPOR | +++ | − | + | |
| JAK2 | +++ | − | +++ | − | |
| JAK1 | − | − | − | − | |
| Tyk2 | − | − | − | nd | |
| Vav | − | − | − | nd | |
| STAT1 | +++ | − | +++ | − | |
| STAT3 | − | − | − | − | |
| STAT5 | +++ | − | ++ | − | |
| SHC | +++ | − | − | +++ | |
| ERK-1 | +++ | +/− | +/− | +++ | |
| JNK | +++ | ++++ | +++++ | +++ | |
| P38 | +++ | − | +++ | nd | |
| PKB/Akt | +++ | − | +++ | ++ | |
| BAD | +++ | − | ++++ | +/− | |
The effects of EPO, different concentrations of orthovanadate, and stem cell factor (SCF) on the physiology and signal transduction of HCD57 were compiled and displayed in this table for reference.
EPO indicates erythropoietin; EPOR, erythropoietin receptor; nd, not determined; −, no activation; +/−, slight activation; +, minor activation; ++, moderate activation; +++, significant activation; ++++, great activation; +++++, greatest activation. For reference, +++ will indicate the level of activation seen in EPO-stimulated HCD57 cells.