Effects of vasostatin and murine interleukin-12 on angiogenesis in vivo
| Additions to Matrigel . | Mean surface area (SEM) occupied by cells (μm2/1.26 × 105 μm2) . | Inhibition (%) . |
|---|---|---|
| None | 373 (67.5) | |
| bFGF | 7337 (1170.4) | |
| bFGF + vasostatin (50 μg/mL) | 3163 (777.7) | 56.9 |
| bFGF + muIL-12 (50 ng/mL) | 4119 (442.4) | 43.9 |
| bFGF + vasostatin (50 μg/mL) + muIL-12 (50 ng/mL) | 1522 (213.4) | 79.3 |
| bFGF + vasostatin (10 μg/mL) | 5528 (338.5) | 24.7 |
| bFGF + muIL-12 (10 ng/mL) | 5205 (363.6) | 29.1 |
| bFGF + vasostatin (10 μg/mL) + muIL-12 (10 ng/mL) | 3059 (442.0) | 58.3 |
| Additions to Matrigel . | Mean surface area (SEM) occupied by cells (μm2/1.26 × 105 μm2) . | Inhibition (%) . |
|---|---|---|
| None | 373 (67.5) | |
| bFGF | 7337 (1170.4) | |
| bFGF + vasostatin (50 μg/mL) | 3163 (777.7) | 56.9 |
| bFGF + muIL-12 (50 ng/mL) | 4119 (442.4) | 43.9 |
| bFGF + vasostatin (50 μg/mL) + muIL-12 (50 ng/mL) | 1522 (213.4) | 79.3 |
| bFGF + vasostatin (10 μg/mL) | 5528 (338.5) | 24.7 |
| bFGF + muIL-12 (10 ng/mL) | 5205 (363.6) | 29.1 |
| bFGF + vasostatin (10 μg/mL) + muIL-12 (10 ng/mL) | 3059 (442.0) | 58.3 |
Mice were injected subcutaneously with Matrigel alone, Matrigel with bFGF (150 ng/mL), Matrigel plus bFGF (150 ng/mL) plus vasostatin (10 or 50 μg/mL), Matrigel plus bFGF (150 ng/mL) plus murine IL-12 (10 or 50 ng/mL), or Matrigel plus bFGF (150 ng/mL) plus vasostatin (10 or 50 μg/mL) plus muIL-12 (10 or 50 ng/mL). Plugs were removed after 7 days, and histologic sections were stained by Masson trichrome. The results reflect the mean surface area (expressed in μm2) occupied by cells within a surface area of 1.26 × 105μm2; 12 to 15 nonoverlapping fields were scanned in each plug; there were 8 plugs per group. Determinations of surface areas were performed by a semiautomated digital analyzer.
bFGF, basic fibroblast growth factor; mu, murine.