Chromosomal abnormalities detected by SKY and FISH in Atm-deficient mouse thymic lymphomas
| Tumor . | Chromosomal aberrations* . | No. gains/losses† . | FISH‡ . | |
|---|---|---|---|---|
| TcrCα/Vα 14D . | IgH 12F . | |||
| AT-1 | T(12;14)1-153 T(14;Dp15)1-155 T(14;Dp3) Is(6;14) | 2/1 | Not done | Not done |
| AT-4 | T(12;14) T(14;Dp15) T(14;X) T(X;Dp11) | 2/2 | LR | LD |
| AT-5 | T(12;14) T(14;Dp15) T(14;DpX) | 2/2 | LR | LD |
| AT-7 | T(12;14) T(Is(1;14);16)1-154 Del(14)# +15 | 1/3 | LR | LD |
| AT-10 | T(12;10) Dp(14) T(X;Dp15) T(17;Dp1) Del(16) −11 | 3/4 | LR | LD |
| AT-11 | T(12;Dp6) Dic(14;14)1-160 Dp(14) Dic(15;Dp15) Rb(16.Dp16)1-164 +15 | 6/1 | LR | LD |
| AT-12 | T(12;9) T(9;Dp15) Del(14) Ace(14)1-161 +10 +13 +15 | 4/3 | LR | LD |
| AT-13 | T(12;14) T(Is(15;14);Dp15) Del(14) | 1/2 | LR | LD |
| Tumor . | Chromosomal aberrations* . | No. gains/losses† . | FISH‡ . | |
|---|---|---|---|---|
| TcrCα/Vα 14D . | IgH 12F . | |||
| AT-1 | T(12;14)1-153 T(14;Dp15)1-155 T(14;Dp3) Is(6;14) | 2/1 | Not done | Not done |
| AT-4 | T(12;14) T(14;Dp15) T(14;X) T(X;Dp11) | 2/2 | LR | LD |
| AT-5 | T(12;14) T(14;Dp15) T(14;DpX) | 2/2 | LR | LD |
| AT-7 | T(12;14) T(Is(1;14);16)1-154 Del(14)# +15 | 1/3 | LR | LD |
| AT-10 | T(12;10) Dp(14) T(X;Dp15) T(17;Dp1) Del(16) −11 | 3/4 | LR | LD |
| AT-11 | T(12;Dp6) Dic(14;14)1-160 Dp(14) Dic(15;Dp15) Rb(16.Dp16)1-164 +15 | 6/1 | LR | LD |
| AT-12 | T(12;9) T(9;Dp15) Del(14) Ace(14)1-161 +10 +13 +15 | 4/3 | LR | LD |
| AT-13 | T(12;14) T(Is(15;14);Dp15) Del(14) | 1/2 | LR | LD |
SKY = spectral karyotyping; FISH = fluorescence in situ hybridization; LR = locus rearranged; LD = locus deleted.
Chromosome rearrangements were detected by SKY analysis of at least 6 metaphase cells per tumor. Aberrations listed were present in at least 5 of the 6 metaphases. Aberrations involving chromosomes 12, 14, and 15 are shown in bold.
The number of partial and whole chromosome gains or losses identified by SKY and FISH.
The results of FISH analysis with bacterial artificial chromosome (BAC) clones to mouse IgH and TcrCα/Vδ loci. As described in the text, all tumors exhibited rearrangement within the Tcr locus on both alleles of chromosome 14 and deletion of the IgH locus on the translocated allele of chromosome 12.
T: translocation. The chromosome donating the centromere is listed first.
Dp: duplication of a chromosome segment.
Is: insertion. The inserted segment is listed first.
#Del: interstitial deletion of a chromosome segment.
Dic: a dicentric chromosome.
Rb: Robertsonian translocation.
Ace: acentric fragment of a chromosome.