Composition of human cell chimerism in the BM of engrafted NOD/SCID mice transplanted with FB, FBM, FL, and CB repopulating cells
| Lineage phenotype . | FB-SRC, % . | FBM-SRC, % . | FL-SRC, % . | CB-SRC, % . |
|---|---|---|---|---|
| CD33+CD15+ | 43 ± 13.8* | 31.5 ± 8.2* | 9 ± 2.1 | 11 ± 6.1 |
| CD33+CD15− | 8.1 ± 4.1 | 16.5 ± 8.1 | 12 ± 1.4 | 4.3 ± 3.8 |
| CD19+CD20+ | 5.8 ± 3.9 | 13 ± 3.6 | 8.3 ± 2.6 | 8.9 ± 4.1 |
| CD19+CD20− | 4.8 ± 3.2* | 17 ± 6.8* | 53 ± 20.1 | 74 ± 12.1 |
| CD34+ | 6.8 ± 3.5 | 6.5 ± 3.7 | 7.2 ± 3.3 | 5.6 ± 2.1 |
| CD34+CD38− | 2.4 ± 0.9* | 0.2 ± 0.1 | 0.8 ± 0.4 | 0.2 ± 0.2 |
| Lineage phenotype . | FB-SRC, % . | FBM-SRC, % . | FL-SRC, % . | CB-SRC, % . |
|---|---|---|---|---|
| CD33+CD15+ | 43 ± 13.8* | 31.5 ± 8.2* | 9 ± 2.1 | 11 ± 6.1 |
| CD33+CD15− | 8.1 ± 4.1 | 16.5 ± 8.1 | 12 ± 1.4 | 4.3 ± 3.8 |
| CD19+CD20+ | 5.8 ± 3.9 | 13 ± 3.6 | 8.3 ± 2.6 | 8.9 ± 4.1 |
| CD19+CD20− | 4.8 ± 3.2* | 17 ± 6.8* | 53 ± 20.1 | 74 ± 12.1 |
| CD34+ | 6.8 ± 3.5 | 6.5 ± 3.7 | 7.2 ± 3.3 | 5.6 ± 2.1 |
| CD34+CD38− | 2.4 ± 0.9* | 0.2 ± 0.1 | 0.8 ± 0.4 | 0.2 ± 0.2 |
Unfractionated or purified human FB, FBM, FL, and CB cells were transplanted into NOD/SCID mice, and murine BM was analyzed after 8 weeks. Human CD45+ cells were gated and analyzed for composition of myeloid (CD33 and CD15), B-lymphoid (CD19 and CD20), and primitive human subsets (CD34 and CD38). The percentage of each lineage is calculated as the mean ± SEM of human cells and was compared between mice transplanted with FB-SRC, FBM-SRC, FL-SRC, or CB-SRC. Data were analyzed by the unpaired, two-tailed Student t test assuming a Gaussian distribution (parametric test) and demonstrated differences as indicated (*); P < .001, n = 3-8.