Percentage of clonogenic progenitors within subpopulations of CD34−CD38−Lin−, CD34+CD38−Lin−, and CD34+CD38+Lin− cells from human FB and CB
| Subpopulation phenotype . | CFU-M, % . | CFU-G, % . | CFU-GM, % . | BFU-E, % . | CFU-GEMM, % . |
|---|---|---|---|---|---|
| Fetal blood | |||||
| CD34−CD38−Lin− | 0.0 ± 0.0 | 27.0 ± 27.0 | 9.5 ± 9.5 | 63.5 ± 36.5 | 0.0 ± 0.0 |
| CD34+CD38−Lin− | 17.3 ± 4.3 | 41.8 ± 14.8 | 3.5 ± 0.5 | 36.7 ± 18.2 | 0.65 ± 0.65 |
| CD34+CD38+Lin− | 9.4 ± 2.3 | 26.7 ± 1.6 | 4.3 ± 1.0 | 59.6 ± 2.9 | 0.0 ± 0.0 |
| Cord blood | |||||
| CD34−CD38−Lin− | 4.3 ± 1.7 | 25.0 ± 7.5 | 0.5 ± 0.5 | 65.0 ± 9.5 | 5.2 ± 0.9 |
| CD34+CD38−Lin− | 9.2 ± 2.7 | 54.9 ± 9.9 | 2.6 ± 1.3 | 30.3 ± 7.4 | 2.9 ± 1.5 |
| CD34+CD38+Lin− | 2.5 ± 0.0 | 51.8 ± 0.0 | 0.0 ± 0.0 | 45.5 ± 0.0 | 0.0 ± 0.0 |
| Subpopulation phenotype . | CFU-M, % . | CFU-G, % . | CFU-GM, % . | BFU-E, % . | CFU-GEMM, % . |
|---|---|---|---|---|---|
| Fetal blood | |||||
| CD34−CD38−Lin− | 0.0 ± 0.0 | 27.0 ± 27.0 | 9.5 ± 9.5 | 63.5 ± 36.5 | 0.0 ± 0.0 |
| CD34+CD38−Lin− | 17.3 ± 4.3 | 41.8 ± 14.8 | 3.5 ± 0.5 | 36.7 ± 18.2 | 0.65 ± 0.65 |
| CD34+CD38+Lin− | 9.4 ± 2.3 | 26.7 ± 1.6 | 4.3 ± 1.0 | 59.6 ± 2.9 | 0.0 ± 0.0 |
| Cord blood | |||||
| CD34−CD38−Lin− | 4.3 ± 1.7 | 25.0 ± 7.5 | 0.5 ± 0.5 | 65.0 ± 9.5 | 5.2 ± 0.9 |
| CD34+CD38−Lin− | 9.2 ± 2.7 | 54.9 ± 9.9 | 2.6 ± 1.3 | 30.3 ± 7.4 | 2.9 ± 1.5 |
| CD34+CD38+Lin− | 2.5 ± 0.0 | 51.8 ± 0.0 | 0.0 ± 0.0 | 45.5 ± 0.0 | 0.0 ± 0.0 |
Progenitor content was measured by enumerating the colony-forming units (CFU) from equal numbers of phenotypically distinct subpopulations isolated from lineage-depleted samples of human fetal blood and cord blood. The number of each type of CFU was determined from plating conditions outlined in “Materials and methods” and identified according to standard protocols. CFU-M indicates CFU-macrophage; CFU-G, CFU-granulocyte; CFU-GM, CFU-granulocyte, macrophage; BFU-E, burst-forming unit-erythroid; and CFU-GEMM, CFU-granulocyte, erythroid, macrophage, megakaryocyte. The percentage of each colony type is calculated as the mean ± SEM.