Ex vivo expansion of Lin−Sca-1+c-kit+ stem cells in the presence of IL-3 or IL-11 do not affect their ability for long-term reconstitution of lymphoid and myeloid cell lineages
| Ex vivo expansion . | Lineage reconstitution, % of Ly5.1+ cells . | ||
|---|---|---|---|
| B220+ . | CD3+ . | Gr-1+/Mac-1+ . | |
| None (control) | 53 ± 2 | 34 ± 3 | 14 ± 2 |
| KFM 116 h | 44 ± 10 | 47 ± 12 | 11 ± 5 |
| KFM3 116 h | 46 ± 9 | 34 ± 5 | 24 ± 10 |
| KFM11 116 h | 55 ± 3 | 33 ± 4 | 14 ± 4 |
| KFM 240 h | 56 ± 5 | 23 ± 1 | 21 ± 4 |
| KFM3 240 h | 52 ± 1 | 33 ± 2 | 15 ± 1 |
| KFM11 240 h | 56 ± 3 | 33 ± 3 | 10 ± 1 |
| Ex vivo expansion . | Lineage reconstitution, % of Ly5.1+ cells . | ||
|---|---|---|---|
| B220+ . | CD3+ . | Gr-1+/Mac-1+ . | |
| None (control) | 53 ± 2 | 34 ± 3 | 14 ± 2 |
| KFM 116 h | 44 ± 10 | 47 ± 12 | 11 ± 5 |
| KFM3 116 h | 46 ± 9 | 34 ± 5 | 24 ± 10 |
| KFM11 116 h | 55 ± 3 | 33 ± 4 | 14 ± 4 |
| KFM 240 h | 56 ± 5 | 23 ± 1 | 21 ± 4 |
| KFM3 240 h | 52 ± 1 | 33 ± 2 | 15 ± 1 |
| KFM11 240 h | 56 ± 3 | 33 ± 3 | 10 ± 1 |
Lethally irradiated C57bl/6 (Ly5.2+) mice were transplanted with 500 or 750 LSK cells freshly isolated (control) or the expansion equivalent of this cell number using various culture conditions, along with 150 000 or 200 000 unfractionated Ly5.2+ BM cells. Peripheral blood cells from transplanted mice were stained and analyzed by flow cytometry 4 months after transplantation for the presence of donor-derived (Ly5.1) cells along with lineage antibodies (B220, B cells; CD3, T cells; Gr-1/Mac-1, myeloid cells). Data are from the same experiments as in Figure 3 and are expressed as mean ± SEM.