IL-3 and IL-11 enhance proliferation but do not affect the level of recruitment of Lin−Sca-1+c-kit+ cells into proliferation in response to KL + FL + MGDF
| . | Clone size . | Total . | ||
|---|---|---|---|---|
| 1 . | 2 . | 3 . | ||
| Medium | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| KFM | 12 (8) | 26 (6) | 39 (1) | 77 (13) |
| KFM3 | 9 (1) | 7 (1) | 63 (11) | 79 (11) |
| KFM11 | 10 (6) | 2 (3) | 62 (12) | 74 (9) |
| . | Clone size . | Total . | ||
|---|---|---|---|---|
| 1 . | 2 . | 3 . | ||
| Medium | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| KFM | 12 (8) | 26 (6) | 39 (1) | 77 (13) |
| KFM3 | 9 (1) | 7 (1) | 63 (11) | 79 (11) |
| KFM11 | 10 (6) | 2 (3) | 62 (12) | 74 (9) |
LSK cells were seeded at 1 cell/well in serum-free medium and cytokines as indicated. After 10 days of incubation, cultures were scored for clonal growth. Size criteria were: I, 2 to 49 cells; II, 50 cells to 10% well covered with cells; III, more than 10% well covered. Results are presented as mean (±SD) from two independent experiments.