Rolling velocities of LS174T and COLO205 cells and neutrophils over platelet P-selectin under flow
| LS174T over platelet P-selectin | 124 ± 9.3 μm/s (n = 27) |
| COLO205 over platelet P-selectin | 56 ± 4.1 μm/s (n = 24) |
| Neutrophils over platelet P-selectin | 4.1 ± 0.9 μm/s (n = 18) |
| LS174T over platelet P-selectin | 124 ± 9.3 μm/s (n = 27) |
| COLO205 over platelet P-selectin | 56 ± 4.1 μm/s (n = 24) |
| Neutrophils over platelet P-selectin | 4.1 ± 0.9 μm/s (n = 18) |
LS174T, COLO205 cells and neutrophils (106/mL) were perfused over a platelet P-selectin substrate (αIIbβ3-blocked platelets) at wall shear stresses of 0.8 dyn/cm2 (LS174T and COLO205 cells) and 1.0 dyn/cm2 (neutrophils). The critical velocity of free-flowing tumor cells adjacent to the wall was experimentally calculated to be about 700 μm/s.42