Table 1. Engraftment of donor-derived... | American Society of Hematology

Table 1.

Engraftment of donor-derived cells in secondary BMT recipients

Primary donor	% donor-derived cells
Primary donor	Bone marrow	Spleen
Normal donor marrow PBS	6.5 ± 4.8	11.9 ± 3.2^*
1	2.3 ± 1.1	8.9 ± 4
2	13.1 ± 6.9	16.3 ± 3.5
3	—	9.1
4	7 ± 5.7	14.2 ± 2.1
5	3.7 ± 0.9	11.2 ± 2.2
Tg04 donor marrow PBS	4.2 ± 3.6^†	5.4 ± 2.8^†
6	10.2 ± 2.7	7.1 ± 3.2
7	2.1 ± 1.1	4.3 ± 1.1
8	0.7	2.0 ± 0.9
9	1.3 ± 0.3	4.0 ± 2.3
10	4.6	4.9
11	6.2	9.9
Tg04 donor marrow MTX	2.9 ± 0.6^‡	3.5 ± 1.1^1-153
12	3.5	3.4
13	2.5	2.0
14	2.7 ± 1	3.4 ± 0.2
15	2.3 ± 0.7	5.1
16	3.6 ± 1.5	3.7 ± 1.7

Primary donor	% donor-derived cells
Primary donor	Bone marrow	Spleen
Normal donor marrow PBS	6.5 ± 4.8	11.9 ± 3.2^*
1	2.3 ± 1.1	8.9 ± 4
2	13.1 ± 6.9	16.3 ± 3.5
3	—	9.1
4	7 ± 5.7	14.2 ± 2.1
5	3.7 ± 0.9	11.2 ± 2.2
Tg04 donor marrow PBS	4.2 ± 3.6^†	5.4 ± 2.8^†
6	10.2 ± 2.7	7.1 ± 3.2
7	2.1 ± 1.1	4.3 ± 1.1
8	0.7	2.0 ± 0.9
9	1.3 ± 0.3	4.0 ± 2.3
10	4.6	4.9
11	6.2	9.9
Tg04 donor marrow MTX	2.9 ± 0.6^‡	3.5 ± 1.1^1-153
12	3.5	3.4
13	2.5	2.0
14	2.7 ± 1	3.4 ± 0.2
15	2.3 ± 0.7	5.1
16	3.6 ± 1.5	3.7 ± 1.7

Bone marrow samples (5 × 10⁶ cells) from primary recipients (described in Figures 3 and 4) that survived 120 days after transplantation were each transplanted into 3 lethally irradiated secondary recipients. Genomic DNA was isolated from these mice 4 months after transplantation, and quantitative Southern analysis was carried out to determine the level of DHFR or APP donor cell engraftment as described in “Materials and methods.” Values are presented as mean ± SD of triplicate secondary recipients (when available) derived from individual primary recipients (listed), and as mean ± SD for all secondary recipients (at the top) for each group. Statistical analysis vs. primary recipients (Fig. 4B):

P < .05;

P < .0001;

P < .00001;

P < .01.

This Feature Is Available To Subscribers Only