Stromal cell coculture maintains JM-1 cell cycle progression
| Treatment . | JM-1 cells recovered (×105/mL) . | % G0/G1 . | % S . | % G2/M . | ||||
|---|---|---|---|---|---|---|---|---|
| NS . | SC . | NS . | SC . | NS . | SC . | NS . | SC . | |
| Control | 12.2 | 15.1 | 56.6 | 45.3 | 34.4 | 44.9 | 9.0 | 9.8 |
| Ara-C | 8.9 | 14.3 | 73.2 | 42.0 | 24.0 | 45.9 | 2.8 | 12.1 |
| VP-16 | 10.0 | 13.7 | 83.2 | 58.0 | 14.7 | 37.7 | 2.1 | 4.3 |
| Treatment . | JM-1 cells recovered (×105/mL) . | % G0/G1 . | % S . | % G2/M . | ||||
|---|---|---|---|---|---|---|---|---|
| NS . | SC . | NS . | SC . | NS . | SC . | NS . | SC . | |
| Control | 12.2 | 15.1 | 56.6 | 45.3 | 34.4 | 44.9 | 9.0 | 9.8 |
| Ara-C | 8.9 | 14.3 | 73.2 | 42.0 | 24.0 | 45.9 | 2.8 | 12.1 |
| VP-16 | 10.0 | 13.7 | 83.2 | 58.0 | 14.7 | 37.7 | 2.1 | 4.3 |
JM-1 cells were collected after treatment with 5 μmol/L VP-16 or 0.1 μg/mL Ara-C for 24 hours in the presence (SC) or absence (NS) of an adherent stromal cell layer. JM-1 cells were fixed in EtOH, treated for 30 minutes with RNase, and stained with propidium iodide for DNA analysis. Data are representative of 2 independent experiments.