Transduction efficiency of HIV-1ΔEN and HIV-1ΔENΔ V3 in macrophages and dendritic cells
. | . | Macrophages . | Dendritic cells . | |
---|---|---|---|---|
Immature . | Mature . | |||
HIV-1ΔEN | MOI 5 | 25.3 ± 5 | 30.7 ± 11 | nt |
HIV-1ΔENΔV3 | MOI 5 | 22.5 ± 4 | 26.5 ± 9 | 5.4 ± 3 |
MOI 50 | nt | 50.5 ± 4 | nt |
. | . | Macrophages . | Dendritic cells . | |
---|---|---|---|---|
Immature . | Mature . | |||
HIV-1ΔEN | MOI 5 | 25.3 ± 5 | 30.7 ± 11 | nt |
HIV-1ΔENΔV3 | MOI 5 | 22.5 ± 4 | 26.5 ± 9 | 5.4 ± 3 |
MOI 50 | nt | 50.5 ± 4 | nt |
Cells were transduced as described in the legend to Figure 2. Dendritic cells (DCs) were transduced either before (immature DCs) or after differentiation (mature DCs). Transduction efficiency was calculated as the percentage of GFP-expressing cells within the total population. Mean ± SD of at least 2 experiments are shown.
MOI, multiplicities of infection; nt, not tested.