Lymphocyte development in wt andLsp1−/− mice in various tissues
| Cell population . | . | Percentage of total cells . | |
|---|---|---|---|
| wt . | Lsp1−/− . | ||
| Bone marrow | |||
| Pro-B cells | B220loCD43+ | 5.4 ± 0.1 (4) | 5.8 ± 0.5 (4) |
| Pre-B cells* | B220loCD43−IgM− | 11.2 ± 0.5 (4) | 9.4 ± 0.7 (4) |
| B cells | |||
| Immature | IgM+IgDlo-neg | 10.3 ± 0.4 (7) | 8.8 ± 0.8 (7) |
| IgM+B220lo | 7.6 ± 0.4 (7) | 7.4 ± 1.0 (7) | |
| Mature | IgM+IgDhi | 3.2 ± 0.2 (7) | 4.4 ± 0.5 (7) |
| IgM+B220hi | 4.3 ± 0.3 (7) | 4.4 ± 0.2 (7) | |
| Thymus | CD4−CD8− | 5.8 ± 0.8 (8) | 5.6 ± 0.4 (9) |
| CD4+CD8+ | 82.1 ± 0.9 (8) | 81.1 ± 0.9 (9) | |
| CD4+CD8− | 9.4 ± 0.3 (8) | 10.3 ± 0.4 (9) | |
| CD4−CD8+ | 2.9 ± 0.1 (8) | 3.0 ± 0.2 (9) | |
| Spleen | |||
| B cells | |||
| Immature | B220+HSAhi | 7.8 ± 0.8 (4) | 6.5 ± 0.3 (4) |
| Mature | B220+HSAlo | 49.6 ± 1.9 (4) | 45.3 ± 0.5 (4) |
| IgM+IgDhi | 45.9 ± 0.8 (7) | 43.1 ± 1.0 (7) | |
| T cells | CD4+CD8− | 24.5 ± 0.9 (7) | 23.9 ± 1.7 (7) |
| CD4−CD8+ | 9.9 ± 1.0 (7) | 10.4 ± 1.6 (7) | |
| α/β TCR+ | 32.7 ± 3.0 (3) | 36.0 ± 8.0 (3) | |
| Blood | |||
| B cells | B220+CD3− | 6.2 ± 0.6 (11) | 7.9 ± 0.8 (10) |
| T cells | B220−CD3+ | 64.8 ± 2.7 (11) | 63.2 ± 3.1 (10) |
| Lymph nodes | |||
| B cells | B220+CD3− | 16.0 ± 1.0 (4) | 14.6 ± 1.0 (5) |
| T cells | B220−CD3+ | 78.1 ± 0.5 (4) | 79.6 ± 1.9 (5) |
| Percentage of lymphocytes | |||
| Peritoneal cavity | |||
| B cells | |||
| B-1a | B220+CD5+ | 49.0 ± 4.0 (5) | 49.1 ± 1.9 (5) |
| Mac-1+CD5+ | 41.9 ± 3.0 (6) | 40.9 ± 4.2 (7) | |
| B-1b + B-2 | B220+CD5− | 27.3 ± 1.3 (5) | 28.8 ± 1.0 (5) |
| T cells | B220−CD5+ | 17.9 ± 4.3 (5) | 12.1 ± 1.8 (5) |
| Cell population . | . | Percentage of total cells . | |
|---|---|---|---|
| wt . | Lsp1−/− . | ||
| Bone marrow | |||
| Pro-B cells | B220loCD43+ | 5.4 ± 0.1 (4) | 5.8 ± 0.5 (4) |
| Pre-B cells* | B220loCD43−IgM− | 11.2 ± 0.5 (4) | 9.4 ± 0.7 (4) |
| B cells | |||
| Immature | IgM+IgDlo-neg | 10.3 ± 0.4 (7) | 8.8 ± 0.8 (7) |
| IgM+B220lo | 7.6 ± 0.4 (7) | 7.4 ± 1.0 (7) | |
| Mature | IgM+IgDhi | 3.2 ± 0.2 (7) | 4.4 ± 0.5 (7) |
| IgM+B220hi | 4.3 ± 0.3 (7) | 4.4 ± 0.2 (7) | |
| Thymus | CD4−CD8− | 5.8 ± 0.8 (8) | 5.6 ± 0.4 (9) |
| CD4+CD8+ | 82.1 ± 0.9 (8) | 81.1 ± 0.9 (9) | |
| CD4+CD8− | 9.4 ± 0.3 (8) | 10.3 ± 0.4 (9) | |
| CD4−CD8+ | 2.9 ± 0.1 (8) | 3.0 ± 0.2 (9) | |
| Spleen | |||
| B cells | |||
| Immature | B220+HSAhi | 7.8 ± 0.8 (4) | 6.5 ± 0.3 (4) |
| Mature | B220+HSAlo | 49.6 ± 1.9 (4) | 45.3 ± 0.5 (4) |
| IgM+IgDhi | 45.9 ± 0.8 (7) | 43.1 ± 1.0 (7) | |
| T cells | CD4+CD8− | 24.5 ± 0.9 (7) | 23.9 ± 1.7 (7) |
| CD4−CD8+ | 9.9 ± 1.0 (7) | 10.4 ± 1.6 (7) | |
| α/β TCR+ | 32.7 ± 3.0 (3) | 36.0 ± 8.0 (3) | |
| Blood | |||
| B cells | B220+CD3− | 6.2 ± 0.6 (11) | 7.9 ± 0.8 (10) |
| T cells | B220−CD3+ | 64.8 ± 2.7 (11) | 63.2 ± 3.1 (10) |
| Lymph nodes | |||
| B cells | B220+CD3− | 16.0 ± 1.0 (4) | 14.6 ± 1.0 (5) |
| T cells | B220−CD3+ | 78.1 ± 0.5 (4) | 79.6 ± 1.9 (5) |
| Percentage of lymphocytes | |||
| Peritoneal cavity | |||
| B cells | |||
| B-1a | B220+CD5+ | 49.0 ± 4.0 (5) | 49.1 ± 1.9 (5) |
| Mac-1+CD5+ | 41.9 ± 3.0 (6) | 40.9 ± 4.2 (7) | |
| B-1b + B-2 | B220+CD5− | 27.3 ± 1.3 (5) | 28.8 ± 1.0 (5) |
| T cells | B220−CD5+ | 17.9 ± 4.3 (5) | 12.1 ± 1.8 (5) |
Values represent the mean percentage ± 1 SEM in total cells of bone marrow, thymus, spleen, blood, and lymph nodes or in total lymphocytes of peritoneal cavity. Numbers in parentheses correspond to the number of mice.
The percentage of pre-B cells in bone marrow was determined for each individual mouse by subtracting the percentage of B220loCD43+ (pro-B) cells from the percentage of B220loIgM− (pro-B + pre-B) cells.