Fold-increase in total cells and Sca-1+ cells in cultures initiated with engrafting phenotypes of Sca-1+lin−AM+ or AM−cells fractionated on the basis of position in cell cycle
Phenotype . | Sca-1+lin− . | CD11a− . | CD43+ . | CD49ddim . | CD49e+ . | CD62L− . | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | |
Total cells3-150 | 1200 | 70 | 1900 | 1400 | 4100 | 100 | 840 | 10 | 3900 | 240 | 4900 | 50 |
Sca1+cells3-151 | 1030 | 35 | 1800 | 1200 | 3970 | 10 | 330 | 0 | 2200 | 33 | 2340 | 0 |
Phenotype . | Sca-1+lin− . | CD11a− . | CD43+ . | CD49ddim . | CD49e+ . | CD62L− . | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | G0/G1 . | S/G2+M . | |
Total cells3-150 | 1200 | 70 | 1900 | 1400 | 4100 | 100 | 840 | 10 | 3900 | 240 | 4900 | 50 |
Sca1+cells3-151 | 1030 | 35 | 1800 | 1200 | 3970 | 10 | 330 | 0 | 2200 | 33 | 2340 | 0 |
AM indicates adhesion molecule.
Fold-increase in total cells was calculated by dividing the cell number on day 14 of cytokine supplemented in vitro culture by the input number on day 0.
Fold-increase in Sca-1+ cells was calculated by multiplying the percentage of Sca-1+ cells on day 14, determined by flow cytometry, by the total number of cells, and then dividing this number by the input number on day 0.