Table 3.

Fold-increase in total cells and Sca-1+ cells in cultures initiated with engrafting phenotypes of Sca-1+linAM+ or AMcells fractionated on the basis of position in cell cycle

PhenotypeSca-1+linCD11aCD43+CD49ddimCD49e+CD62L
G0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+M
Total cells3-150 1200 70 1900 1400 4100 100 840 10 3900 240 4900 50 
Sca1+cells3-151 1030 35 1800 1200 3970 10 330 2200 33 2340 
PhenotypeSca-1+linCD11aCD43+CD49ddimCD49e+CD62L
G0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+MG0/G1S/G2+M
Total cells3-150 1200 70 1900 1400 4100 100 840 10 3900 240 4900 50 
Sca1+cells3-151 1030 35 1800 1200 3970 10 330 2200 33 2340 

AM indicates adhesion molecule.

F3-150

Fold-increase in total cells was calculated by dividing the cell number on day 14 of cytokine supplemented in vitro culture by the input number on day 0.

F3-151

Fold-increase in Sca-1+ cells was calculated by multiplying the percentage of Sca-1+ cells on day 14, determined by flow cytometry, by the total number of cells, and then dividing this number by the input number on day 0.

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