Table 2. HPP-CFC and LPP-CFC content and... | American Society of Hematology

Table 2.

HPP-CFC and LPP-CFC content and proliferative potential of Sca-1⁺lin⁻AM⁺ or AM⁻cells

	Sca-1⁺Lin⁻	CD11a		CD43		CD44		CD49d		CD49e		CD62L
	Sca-1⁺Lin⁻	Pos	Neg	Pos	Neg	Bright	Dim	Bright	Dim	Pos	Neg	Pos	Neg
HPP-CFC^*	100	6	126	58	0	45	86	71	88	186	4	59	106
LPP-CFC^*	100	10	115	47	1	35	55	57	58	148	9	37	69
Proliferative potential^†	Nd	440	650	480	360	290	190	200	650	230	15	110	570

	Sca-1⁺Lin⁻	CD11a		CD43		CD44		CD49d		CD49e		CD62L
	Sca-1⁺Lin⁻	Pos	Neg	Pos	Neg	Bright	Dim	Bright	Dim	Pos	Neg	Pos	Neg
HPP-CFC^*	100	6	126	58	0	45	86	71	88	186	4	59	106
LPP-CFC^*	100	10	115	47	1	35	55	57	58	148	9	37	69
Proliferative potential^†	Nd	440	650	480	360	290	190	200	650	230	15	110	570

HPP-CFC indicates high proliferative potential–colony forming cells; and LPP-CFC, low proliferative potential-colony forming cells.

HPP-CFC and LPP-CFC data are normalized by assigning a value of 100% to the HPP-CFC and LPP-CFC content of Sca-1⁺lin⁻ cells in each experiment, and adjusting remaining values accordingly. Data are means from 3 separate experiments. For ease of presentation, SEM values are excluded from the table, but range from 0 to 35 for HPP-CFC data, and from 0 to 27 for LPP-CFC data.

†

Proliferative potential is expressed as fold-increase in total cells from day 0 to day 11. AM⁺ and AM⁻Sca-1⁺lin⁻ cells were cultured in vitro with mSCF, mIL1α, mIL3, hIL6, and hFlt3-L for 11 days. Fold-increase was calculated by dividing the cell number on day 11 by the input number on day 0. Data shown are from 1 of 2 experiments with similar results.

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