Table 2. Functional CTL activity of in... | American Society of Hematology

Table 2.

Functional CTL activity of in vitro expanded cells correlates with tetrameric Mamu-A^*01/p11C, C-M complex binding to CD8⁺ T cells in different secondary lymphoid compartments of SIVmac-infected, Mamu-A^*01+ rhesus monkeys

Monkey	Spleen			Mesenteric lymph node			PBL
	Fresh	p11C, C-M^*expanded		Fresh	p11C, C-M expanded		Fresh	p11C, C-M expanded
	% tetramer	% tetramer	% lysis (5/3/1)^†	% tetramer	% tetramer	% lysis (5/3/1)	% tetramer	% tetramer	% lysis (5/3/1)
575	8.7^‡	8.3^2-153	29/21/15^2-153	2.7	28.8	34/25/15	5.3	18.3	14/9/5
3KI	3.0	49.0	55/50/40	2.8	43.9	57/51/40	1.3	61.3	67/67/60
IPI	9.4	49.8	55/54/36	7.7	69.5	58/52/46	6.0	69.5	55/53/45
297	11.7	19.5	18/12/6	6.2	6.5	18/14/7	6.2	50.3	51/42/31

Monkey	Spleen			Mesenteric lymph node			PBL
	Fresh	p11C, C-M^*expanded		Fresh	p11C, C-M expanded		Fresh	p11C, C-M expanded
	% tetramer	% tetramer	% lysis (5/3/1)^†	% tetramer	% tetramer	% lysis (5/3/1)	% tetramer	% tetramer	% lysis (5/3/1)
575	8.7^‡	8.3^2-153	29/21/15^2-153	2.7	28.8	34/25/15	5.3	18.3	14/9/5
3KI	3.0	49.0	55/50/40	2.8	43.9	57/51/40	1.3	61.3	67/67/60
IPI	9.4	49.8	55/54/36	7.7	69.5	58/52/46	6.0	69.5	55/53/45
297	11.7	19.5	18/12/6	6.2	6.5	18/14/7	6.2	50.3	51/42/31

p11C, C-M represents the 9-amino acid fragment optimal epitope of the SIV Gag 12-amino acid peptide p11C.

†

E:T ratios.

‡

Percent staining of tetrameric Mamu-A*01/p11C, C-M complex on CD8⁺ T cells. CD8⁺ T cells are defined by gating on CD8αβ⁺ CD3⁺ cells.

F2-153

Percent p11C, C-M-specific lysis was calculated as percent specific release by p11C, C-M-pulsed Mamu-A*01+ target cells minus the percent specific release by control peptide p11B-pulsedMamu-A*01+ target cells.

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