Inhibition of IL-2-induced NK cell cytotoxicity by GR and its reversal by histamine
| Experiment no. . | Cells . | Treatment* . | |||
|---|---|---|---|---|---|
| Medium . | Histamine . | IL-2 . | Histamine + IL-2 . | ||
| I | Lymph | 54 ± 3 | 53 ± 3 | 60 ± 3 | 66 ± 3 |
| Lymph + GR (80)† | 9 ± 1 | 23 ± 2 | 21 ± 3 | 48 ± 1 | |
| GR (80) | <3 | <3 | <3 | <3 | |
| II | Lymph | 48 ± 2 | 45 ± 3 | 62 ± 4 | 63 ± 2 |
| Lymph + GR (80) | 15 ± 2 | 30 ± 3 | 14 ± 3 | 47 ± 5 | |
| GR (80) | <3 | <3 | <3 | <3 | |
| Experiment no. . | Cells . | Treatment* . | |||
|---|---|---|---|---|---|
| Medium . | Histamine . | IL-2 . | Histamine + IL-2 . | ||
| I | Lymph | 54 ± 3 | 53 ± 3 | 60 ± 3 | 66 ± 3 |
| Lymph + GR (80)† | 9 ± 1 | 23 ± 2 | 21 ± 3 | 48 ± 1 | |
| GR (80) | <3 | <3 | <3 | <3 | |
| II | Lymph | 48 ± 2 | 45 ± 3 | 62 ± 4 | 63 ± 2 |
| Lymph + GR (80) | 15 ± 2 | 30 ± 3 | 14 ± 3 | 47 ± 5 | |
| GR (80) | <3 | <3 | <3 | <3 | |
Data are NK cell cytotoxicity against K 562 target cells (mean ± SEM of sextuplicate determinations). Results obtained using NK cell-enriched lymphocytes (lymph) and GR in two experiments are shown. Final histamine concentration was 10 μmol/L, IL-2 100 U/mL.
Numbers in parentheses refer to GR added × 103.