Preparative purification of SakSTAR variants fromEscherichia coli cytosolic extracts
| Compound . | Code . | Protein (mg) . | Specific activity (kU/mg) . | Endotoxin (EU/mg) . | Clp (mL · min−1) . | C50 (μg/kg) . |
|---|---|---|---|---|---|---|
| SakSTAR | — | 2400 | 150 | 2.9 | 2.2 | 23 |
| SakSTAR(K74Q,E80A,D82A,K130T,K135R) | SY15 | 840 | 140 | <0.1 | 4.1 | 22 |
| SakSTAR(E65D,K74R,E80A,D82A,K130T,K135R) | SY19 | 800 | 150 | 0.3 | 3.7 | 28 |
| SakSTAR(K35A,E65D,K74Q,E80A,D82A,K130T,K135R) | SY46 | 340 | 140 | <0.1 | 1.6 | 18 |
| SakSTAR(E65Q,K74Q,D82A,S84A,E108A,K109A,K130T, K135R,K136A,∇137K) | SY118 | 690 | 1400 | <0.1 | 0.6 | 18 |
| SakSTAR(K35A,E65Q,K74Q,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R,K136A,∇137K) | SY141 | 110 | 2700 | <0.1 | 1.0 | 19 |
| SakSTAR(K35A,E65Q,K74R,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R,K136A,∇137K) | SY145 | 200 | 5900 | <0.1 | 0.8 | 29 |
| SakSTAR(K35A,E65Q,K74Q,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R) | SY151 | 630 | 170 | 0.1 | 0.6 | 19 |
| SakSTAR(K35A,E65Q,K74R,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R) | SY155 | 76 | 180 | 0.3 | 1.6 | 28 |
| Compound . | Code . | Protein (mg) . | Specific activity (kU/mg) . | Endotoxin (EU/mg) . | Clp (mL · min−1) . | C50 (μg/kg) . |
|---|---|---|---|---|---|---|
| SakSTAR | — | 2400 | 150 | 2.9 | 2.2 | 23 |
| SakSTAR(K74Q,E80A,D82A,K130T,K135R) | SY15 | 840 | 140 | <0.1 | 4.1 | 22 |
| SakSTAR(E65D,K74R,E80A,D82A,K130T,K135R) | SY19 | 800 | 150 | 0.3 | 3.7 | 28 |
| SakSTAR(K35A,E65D,K74Q,E80A,D82A,K130T,K135R) | SY46 | 340 | 140 | <0.1 | 1.6 | 18 |
| SakSTAR(E65Q,K74Q,D82A,S84A,E108A,K109A,K130T, K135R,K136A,∇137K) | SY118 | 690 | 1400 | <0.1 | 0.6 | 18 |
| SakSTAR(K35A,E65Q,K74Q,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R,K136A,∇137K) | SY141 | 110 | 2700 | <0.1 | 1.0 | 19 |
| SakSTAR(K35A,E65Q,K74R,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R,K136A,∇137K) | SY145 | 200 | 5900 | <0.1 | 0.8 | 29 |
| SakSTAR(K35A,E65Q,K74Q,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R) | SY151 | 630 | 170 | 0.1 | 0.6 | 19 |
| SakSTAR(K35A,E65Q,K74R,D82A,S84A,T90A,E99D,T101S, E108A,K109A,K130T,K135R) | SY155 | 76 | 180 | 0.3 | 1.6 | 28 |